Purification, characterization, and molecular cloning of a novel rat liver Dopa/tyrosine sulfotransferase

Yoichi Sakakibara, Yasunari Takami, Christian W Zwieb, Tatsuo Nakayama, Masahito Suiko, Hiroshi Nakajima, Ming Cheh Liu

Research output: Contribution to journalArticle

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Abstract

A novel sulfotransferase was purified from the rat liver cytosol to electrophoretic homogeneity via five column chromatography steps (hydroxylapatite I, DEAE Bio-Gel, ATP-agarose I, hydroxylapatite II, and ATP- agarose II). The minimum molecular weight of the purified enzyme was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to be ~33,000. Gel filtration chromatography revealed a native molecular weight of 34,000, indicating the enzyme being present in the monomeric form. The purified sulfotransferase displayed enzymatic activities, with a pH optimum of 9.25, toward various tyrosine and 3,4-dihydroxyphenyl-alanine (Dopa) isomers, except DL-ortho-tyrosine. Thyroid hormones, as well as dopamine and p-nitrophenol, could also be used as substrates. The apparent K(m) value of the enzyme (designated the Dopa/tyrosine sulfotransferase) for L-Dopa, determined at a constant 14 μM of 3'-phosphoadenosine 5'-phosphosulfate, was 0.76 mM. The intact enzyme was found to be N-blocked when subjected to N- terminal sequencing. Three internal partial amino acid sequences, obtained by analyzing its proteolytic fragments, were found to be distinct from the homologous sequences of other known rat liver sulfotransferases. The deduced amino acid sequence of a full-length cDNA isolated from a rat liver cDNA library confirmed the identity of the Dopa/tyrosine sulfotransferase as a new type of aryl sulfotransferase. Upon transfection of COS-7 cells with an expression vector (pMSG-CMV) harboring the full-length cDNA, a 33-kDa protein displaying enzymatic and immunological properties similar to those of the purified Dopa/tyrosine sulfotransferase was expressed.

Original languageEnglish (US)
Pages (from-to)30470-30478
Number of pages9
JournalJournal of Biological Chemistry
Volume270
Issue number51
DOIs
StatePublished - Dec 22 1995
Externally publishedYes

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Sulfotransferases
Cloning
Molecular Cloning
Alanine
Liver
Purification
Tyrosine
Rats
Enzymes
Durapatite
Amino Acid Sequence
Arylsulfotransferase
Complementary DNA
Molecular Weight
Gels
Molecular weight
Phosphoadenosine Phosphosulfate
Amino Acids
Column chromatography
COS Cells

ASJC Scopus subject areas

  • Biochemistry

Cite this

Sakakibara, Y., Takami, Y., Zwieb, C. W., Nakayama, T., Suiko, M., Nakajima, H., & Liu, M. C. (1995). Purification, characterization, and molecular cloning of a novel rat liver Dopa/tyrosine sulfotransferase. Journal of Biological Chemistry, 270(51), 30470-30478. https://doi.org/10.1074/jbc.270.51.30470

Purification, characterization, and molecular cloning of a novel rat liver Dopa/tyrosine sulfotransferase. / Sakakibara, Yoichi; Takami, Yasunari; Zwieb, Christian W; Nakayama, Tatsuo; Suiko, Masahito; Nakajima, Hiroshi; Liu, Ming Cheh.

In: Journal of Biological Chemistry, Vol. 270, No. 51, 22.12.1995, p. 30470-30478.

Research output: Contribution to journalArticle

Sakakibara, Y, Takami, Y, Zwieb, CW, Nakayama, T, Suiko, M, Nakajima, H & Liu, MC 1995, 'Purification, characterization, and molecular cloning of a novel rat liver Dopa/tyrosine sulfotransferase', Journal of Biological Chemistry, vol. 270, no. 51, pp. 30470-30478. https://doi.org/10.1074/jbc.270.51.30470
Sakakibara, Yoichi ; Takami, Yasunari ; Zwieb, Christian W ; Nakayama, Tatsuo ; Suiko, Masahito ; Nakajima, Hiroshi ; Liu, Ming Cheh. / Purification, characterization, and molecular cloning of a novel rat liver Dopa/tyrosine sulfotransferase. In: Journal of Biological Chemistry. 1995 ; Vol. 270, No. 51. pp. 30470-30478.
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