Purification and partial characterization of ring-shaped miniparticles

Fred R. Harmon, William H. Spohn, Naochika Domae, Chul S Ha, Harris Busch

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Small ring-shaped miniparticles, found in the nucleus nucleolus and cytoplasm of a variety of normal and malignant cells, have been purified by a procedure involving differential and gradient centrifugation, hydroxylapatite chromatography and preparative electrophoresis. They are multisubunit proteins comprised of at least six polypeptide chains of Mr 20,000-30,000 Daltons (by one-D SDS-PAGE which present a series of 20-30 spots on two-D IEF/SDS gels. They can be reversibly dissociated in EDTA containing buffers. They are not membrane associated and contain no carbohydrate. They have an S20,W of 21S on sucrose gradients. They are immunologically similar in several human cell lines.

Original languageEnglish (US)
Pages (from-to)333-343
Number of pages11
JournalCell Biology International Reports
Volume7
Issue number5
DOIs
StatePublished - 1983
Externally publishedYes

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Durapatite
Centrifugation
Edetic Acid
Sucrose
Electrophoresis
Chromatography
Polyacrylamide Gel Electrophoresis
Buffers
Cytoplasm
Gels
Carbohydrates
Cell Line
Peptides
Membranes
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

Purification and partial characterization of ring-shaped miniparticles. / Harmon, Fred R.; Spohn, William H.; Domae, Naochika; Ha, Chul S; Busch, Harris.

In: Cell Biology International Reports, Vol. 7, No. 5, 1983, p. 333-343.

Research output: Contribution to journalArticle

Harmon, Fred R. ; Spohn, William H. ; Domae, Naochika ; Ha, Chul S ; Busch, Harris. / Purification and partial characterization of ring-shaped miniparticles. In: Cell Biology International Reports. 1983 ; Vol. 7, No. 5. pp. 333-343.
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