Pulsed-field gel electrophoresis as as epidemiologic tool for enterococci and streptococci

Jan E. Patterson, Cindy C. Kelly

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Enterococci (Enterococcus faecium and Enterococcus faecalis) and streptococci such as Streptococcus pyogenes (Group A streptococcus), Streptococcus agalactiae (Group B streptococcus), and Streptococcus pneumoniae are increasing in importance as both hospital-acquired and community pathogens. Emerging resistance and increasing incidence of these organisms has necessitated the analysis of their epidemiologic mechanisms of spread. Pulsed-field gel electrophoresis (PFGE) has emerged as the one of the most widely applicable, reproducible, and stable methods to examine strain identity in bacterial organisms. The procedure used in our laboratory for PFGE typing of whole cell DNA digested with SmaI for enterococci, S. pneumoniae, S. pyogenes, and S. agalacatiae is presented. Issues regarding interpretation are also reviewed and discussed.

Original languageEnglish (US)
Pages (from-to)233-239
Number of pages7
JournalMethods in Cell Science
Volume20
Issue number1-4
DOIs
StatePublished - Dec 1 1998

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Keywords

  • Enterococci
  • Epidemiologic typing
  • Pulsed-field gel electrophoresis
  • Streptococci

ASJC Scopus subject areas

  • Cell Biology

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