Abstract
Two experimental animal models exhibiting functional and morphologic changes of exocrine glands similar to those seen in patients with cystic fibrosis (CF) have been reported in the rat: chronic stimulation with reserpine (Martinez et al. 1973 Pediatr. Res. 9:463, 470) and chronic stimulation with isoprenaline (Sturgess and Reid 1973 Br. J. Exp. Pathol. 54:388). We have studied the pulmonary function of these models induced by injecting rats subcutaneously with reserpine (RES, 0.5 mg/kg/day), isoproterenol (ISO, 25 mg/kg/day), or saline (Con, 1.0 ml/kg/day) for 6 days. Plethysmographic measurements were made for functional residual capacity (FRC), airways resistance (R(aw)), specific airways conductance (sG(aw)), phase difference between air flow rate and mean alveolar pressure (PD), frequency of breathing (f), and tidal volume (V(T)) of the anesthetized rats. In the RES and ISO rats, the FRC, R(aw) and f were not different from Con values. The PD was greater and the V(T) was less than Con values (p < 0.05). The results of both studies indicate uneven ventilation (increased PD) and penduluft (decreased V(T)) consistent with maldistribution of resistance and/or compliances of the peripheral airways and/or alveolar compartments. These physiologic effects can be related to the morphologic changes reported for the airways of rats under chronic adrenergic stimulation (ISO) and expected for rats under chronic catecholamines depletion (RES). Since peripheral airways involvement is usually the earliest pulmonary lesion found in CF, these studies indicate that the RES and ISO models may be representative of the early pulmonary involvement of CF.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1028-1031 |
| Number of pages | 4 |
| Journal | Pediatric Research |
| Volume | 18 |
| Issue number | 10 |
| State | Published - 1984 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Pediatrics, Perinatology, and Child Health
Cite this
Pulmonary function of the reserpine and isoproterenol models of cystic fibrosis. / Boyd, R. L.; Francis, E. M.; Fletcher, M. T.; Mangos, J. A.
In: Pediatric Research, Vol. 18, No. 10, 1984, p. 1028-1031.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Pulmonary function of the reserpine and isoproterenol models of cystic fibrosis
AU - Boyd, R. L.
AU - Francis, E. M.
AU - Fletcher, M. T.
AU - Mangos, J. A.
PY - 1984
Y1 - 1984
N2 - Two experimental animal models exhibiting functional and morphologic changes of exocrine glands similar to those seen in patients with cystic fibrosis (CF) have been reported in the rat: chronic stimulation with reserpine (Martinez et al. 1973 Pediatr. Res. 9:463, 470) and chronic stimulation with isoprenaline (Sturgess and Reid 1973 Br. J. Exp. Pathol. 54:388). We have studied the pulmonary function of these models induced by injecting rats subcutaneously with reserpine (RES, 0.5 mg/kg/day), isoproterenol (ISO, 25 mg/kg/day), or saline (Con, 1.0 ml/kg/day) for 6 days. Plethysmographic measurements were made for functional residual capacity (FRC), airways resistance (R(aw)), specific airways conductance (sG(aw)), phase difference between air flow rate and mean alveolar pressure (PD), frequency of breathing (f), and tidal volume (V(T)) of the anesthetized rats. In the RES and ISO rats, the FRC, R(aw) and f were not different from Con values. The PD was greater and the V(T) was less than Con values (p < 0.05). The results of both studies indicate uneven ventilation (increased PD) and penduluft (decreased V(T)) consistent with maldistribution of resistance and/or compliances of the peripheral airways and/or alveolar compartments. These physiologic effects can be related to the morphologic changes reported for the airways of rats under chronic adrenergic stimulation (ISO) and expected for rats under chronic catecholamines depletion (RES). Since peripheral airways involvement is usually the earliest pulmonary lesion found in CF, these studies indicate that the RES and ISO models may be representative of the early pulmonary involvement of CF.
AB - Two experimental animal models exhibiting functional and morphologic changes of exocrine glands similar to those seen in patients with cystic fibrosis (CF) have been reported in the rat: chronic stimulation with reserpine (Martinez et al. 1973 Pediatr. Res. 9:463, 470) and chronic stimulation with isoprenaline (Sturgess and Reid 1973 Br. J. Exp. Pathol. 54:388). We have studied the pulmonary function of these models induced by injecting rats subcutaneously with reserpine (RES, 0.5 mg/kg/day), isoproterenol (ISO, 25 mg/kg/day), or saline (Con, 1.0 ml/kg/day) for 6 days. Plethysmographic measurements were made for functional residual capacity (FRC), airways resistance (R(aw)), specific airways conductance (sG(aw)), phase difference between air flow rate and mean alveolar pressure (PD), frequency of breathing (f), and tidal volume (V(T)) of the anesthetized rats. In the RES and ISO rats, the FRC, R(aw) and f were not different from Con values. The PD was greater and the V(T) was less than Con values (p < 0.05). The results of both studies indicate uneven ventilation (increased PD) and penduluft (decreased V(T)) consistent with maldistribution of resistance and/or compliances of the peripheral airways and/or alveolar compartments. These physiologic effects can be related to the morphologic changes reported for the airways of rats under chronic adrenergic stimulation (ISO) and expected for rats under chronic catecholamines depletion (RES). Since peripheral airways involvement is usually the earliest pulmonary lesion found in CF, these studies indicate that the RES and ISO models may be representative of the early pulmonary involvement of CF.
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UR - http://www.scopus.com/inward/citedby.url?scp=0021150554&partnerID=8YFLogxK
M3 - Article
C2 - 6493846
AN - SCOPUS:0021150554
VL - 18
SP - 1028
EP - 1031
JO - Pediatric Research
JF - Pediatric Research
SN - 0031-3998
IS - 10
ER -