Pulmonary function changes in rats with taurocholate-induced pancreatitis are attenuated by pretreatment with melatonin

Ting Ywan Chou, Russel J Reiter, Kuan Hao Chen, Fur Jiang Leu, David Wang, Diana Y. Yeh

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Melatonin is a free radical scavenger and broad-spectrum antioxidant with immunomodulatory effects. We studied the effects of melatonin on changes in lung function, oxidative/nitrosative stress, and inflammatory cell sequestration in an acute pancreatitis (AP)-associated lung inflammation model. Acute pancreatitis was induced by injection of 5% sodium taurocholate into the pancreatic duct of rats. Animals were randomized into control, AP, and a melatonin pretreatment (10 mg/kg)/AP group. Functional residual capacity (FRC), lung compliance (Cchord), expiratory flow rate at 50% (FEF50), airway resistance index (RI), and peak expiratory flow rate (PEF) were evaluated. White blood cell count (WBC) and hydrogen peroxide, lung lavage fluid WBC, methylguanidine, protein, lactic dehydrogenase (LDH), nitric oxide (NO), and leukotriene B4 (LTB4) levels were determined. Lung wet-to-dry weight ratio, peroxynitrite, and inducible nitric oxide synthase (NOS) mRNA and protein were measured. AP induction resulted in reductions in FRC, Cchord, FEF50, and PEF, and increase in RI and lung wet-to-dry weight ratio. Blood and lung lavage fluid WBC, lavage fluid LDH, protein, and blood hydrogen peroxide also increased. Levels of hydroxyl radicals, nitric oxide, and LTB4 in lung lavage fluid, inducible NOS mRNA, protein expression, and peroxynitrite in lung tissue also were significantly elevated. Pretreatment with melatonin attenuated obstructive and restrictive ventilatory insufficiency induced by AP. Blood and lavage WBC, lavage LDH and protein, lung edema, oxidative/nitrosative stress, and lipoxygenase pathway derivatives were also significantly attenuated by melatonin. We conclude that melatonin decreases AP-induced obstructive and restrictive lung function changes via its antioxidant and anti-inflammatory properties.

Original languageEnglish (US)
Pages (from-to)196-203
Number of pages8
JournalJournal of Pineal Research
Volume56
Issue number2
DOIs
StatePublished - Mar 2014

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Taurocholic Acid
Melatonin
Pancreatitis
Lung
Leukocyte Count
Therapeutic Irrigation
Bronchoalveolar Lavage Fluid
Functional Residual Capacity
Peak Expiratory Flow Rate
Oxidoreductases
Milk
Peroxynitrous Acid
Leukotriene B4
Nitric Oxide Synthase Type II
Pulmonary Edema
Hydrogen Peroxide
Nitric Oxide
Proteins
Oxidative Stress
Methylguanidine

Keywords

  • lung function
  • melatonin
  • nitrosative stress
  • oxidative stress
  • pancreatitis

ASJC Scopus subject areas

  • Endocrinology

Cite this

Pulmonary function changes in rats with taurocholate-induced pancreatitis are attenuated by pretreatment with melatonin. / Chou, Ting Ywan; Reiter, Russel J; Chen, Kuan Hao; Leu, Fur Jiang; Wang, David; Yeh, Diana Y.

In: Journal of Pineal Research, Vol. 56, No. 2, 03.2014, p. 196-203.

Research output: Contribution to journalArticle

Chou, Ting Ywan ; Reiter, Russel J ; Chen, Kuan Hao ; Leu, Fur Jiang ; Wang, David ; Yeh, Diana Y. / Pulmonary function changes in rats with taurocholate-induced pancreatitis are attenuated by pretreatment with melatonin. In: Journal of Pineal Research. 2014 ; Vol. 56, No. 2. pp. 196-203.
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