Pueraria mirifica extract and puerarin enhance proliferation and expression of alkaline phosphatase and type i collagen in primary baboon osteoblasts

Wacharaporn Tiyasatkulkovit, Suchinda Malaivijitnond, Narattaphol Charoenphandhu, Lorena M. Havill, Allen L. Ford, John L. Vandeberg

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Phytoestrogen-rich Pueraria mirifica (PM) tuberous extract is a promising candidate for the development of anti-osteoporosis drugs for postmenopausal women, but its action has never been validated in humans or in non-human primates, which are more closely related to humans than rodents. In vitro study of non-human primate osteoblasts is thus fundamental to prepare for in vivo studies of phytoestrogen effects on primate bone. This study aimed to establish a culture system of baboon primary osteoblasts and to investigate the effects of PM extract and its phytoestrogens on these cells. Primary osteoblasts from adult baboon fibulae exhibited osteoblast characteristics in regard to proliferation, differentiation, mineralization, and estrogen receptor expression. They responded to 17β-estradiol by increased proliferation rate and mRNA levels of alkaline phosphatase (ALP), type I collagen, and osteocalcin. After being exposed for 48 h to 100 μg/ml PM extract, 1000 nM genistein, or 1000 nM puerarin, primary baboon osteoblasts markedly increased the rate of proliferation and mRNA levels of ALP and type I collagen without changes in Runx2, osterix, or osteocalcin expression. PM extract, genistein, and puerarin also decreased the RANKL/OPG ratio, suggesting that they could decrease osteoclast-mediated bone resorption. However, neither PM extract nor its phytoestrogens altered calcium deposition in osteoblast culture. In conclusion, we have established baboon primary osteoblast culture, which is a new tool for bone research and drug discovery. Furthermore, the present results provide substantial support for the potential of PM extract and its phytoestrogens to be developed as therapeutic agents against bone fragility.

Original languageEnglish (US)
Pages (from-to)1498-1503
Number of pages6
JournalPhytomedicine
Volume21
Issue number12
DOIs
StatePublished - Oct 15 2014
Externally publishedYes

Fingerprint

Pueraria
Papio
Osteoblasts
Phytoestrogens
Alkaline Phosphatase
Collagen
Primates
Genistein
Osteocalcin
Collagen Type I
Bone and Bones
Messenger RNA
Fibula
Postmenopausal Osteoporosis
Osteoclasts
Drug Discovery
Bone Resorption
puerarin
Estrogen Receptors
Estradiol

Keywords

  • Baboon
  • Genistein
  • Osteoblasts
  • Osteoporosis
  • Pueraria mirifica
  • Puerarin

ASJC Scopus subject areas

  • Drug Discovery
  • Pharmacology
  • Pharmaceutical Science
  • Complementary and alternative medicine
  • Molecular Medicine
  • Medicine(all)

Cite this

Tiyasatkulkovit, W., Malaivijitnond, S., Charoenphandhu, N., Havill, L. M., Ford, A. L., & Vandeberg, J. L. (2014). Pueraria mirifica extract and puerarin enhance proliferation and expression of alkaline phosphatase and type i collagen in primary baboon osteoblasts. Phytomedicine, 21(12), 1498-1503. https://doi.org/10.1016/j.phymed.2014.06.019

Pueraria mirifica extract and puerarin enhance proliferation and expression of alkaline phosphatase and type i collagen in primary baboon osteoblasts. / Tiyasatkulkovit, Wacharaporn; Malaivijitnond, Suchinda; Charoenphandhu, Narattaphol; Havill, Lorena M.; Ford, Allen L.; Vandeberg, John L.

In: Phytomedicine, Vol. 21, No. 12, 15.10.2014, p. 1498-1503.

Research output: Contribution to journalArticle

Tiyasatkulkovit, W, Malaivijitnond, S, Charoenphandhu, N, Havill, LM, Ford, AL & Vandeberg, JL 2014, 'Pueraria mirifica extract and puerarin enhance proliferation and expression of alkaline phosphatase and type i collagen in primary baboon osteoblasts', Phytomedicine, vol. 21, no. 12, pp. 1498-1503. https://doi.org/10.1016/j.phymed.2014.06.019
Tiyasatkulkovit, Wacharaporn ; Malaivijitnond, Suchinda ; Charoenphandhu, Narattaphol ; Havill, Lorena M. ; Ford, Allen L. ; Vandeberg, John L. / Pueraria mirifica extract and puerarin enhance proliferation and expression of alkaline phosphatase and type i collagen in primary baboon osteoblasts. In: Phytomedicine. 2014 ; Vol. 21, No. 12. pp. 1498-1503.
@article{278caed67d644066bcb97988163a1fa8,
title = "Pueraria mirifica extract and puerarin enhance proliferation and expression of alkaline phosphatase and type i collagen in primary baboon osteoblasts",
abstract = "Phytoestrogen-rich Pueraria mirifica (PM) tuberous extract is a promising candidate for the development of anti-osteoporosis drugs for postmenopausal women, but its action has never been validated in humans or in non-human primates, which are more closely related to humans than rodents. In vitro study of non-human primate osteoblasts is thus fundamental to prepare for in vivo studies of phytoestrogen effects on primate bone. This study aimed to establish a culture system of baboon primary osteoblasts and to investigate the effects of PM extract and its phytoestrogens on these cells. Primary osteoblasts from adult baboon fibulae exhibited osteoblast characteristics in regard to proliferation, differentiation, mineralization, and estrogen receptor expression. They responded to 17β-estradiol by increased proliferation rate and mRNA levels of alkaline phosphatase (ALP), type I collagen, and osteocalcin. After being exposed for 48 h to 100 μg/ml PM extract, 1000 nM genistein, or 1000 nM puerarin, primary baboon osteoblasts markedly increased the rate of proliferation and mRNA levels of ALP and type I collagen without changes in Runx2, osterix, or osteocalcin expression. PM extract, genistein, and puerarin also decreased the RANKL/OPG ratio, suggesting that they could decrease osteoclast-mediated bone resorption. However, neither PM extract nor its phytoestrogens altered calcium deposition in osteoblast culture. In conclusion, we have established baboon primary osteoblast culture, which is a new tool for bone research and drug discovery. Furthermore, the present results provide substantial support for the potential of PM extract and its phytoestrogens to be developed as therapeutic agents against bone fragility.",
keywords = "Baboon, Genistein, Osteoblasts, Osteoporosis, Pueraria mirifica, Puerarin",
author = "Wacharaporn Tiyasatkulkovit and Suchinda Malaivijitnond and Narattaphol Charoenphandhu and Havill, {Lorena M.} and Ford, {Allen L.} and Vandeberg, {John L.}",
year = "2014",
month = "10",
day = "15",
doi = "10.1016/j.phymed.2014.06.019",
language = "English (US)",
volume = "21",
pages = "1498--1503",
journal = "Phytomedicine",
issn = "0944-7113",
publisher = "Urban und Fischer Verlag Jena",
number = "12",

}

TY - JOUR

T1 - Pueraria mirifica extract and puerarin enhance proliferation and expression of alkaline phosphatase and type i collagen in primary baboon osteoblasts

AU - Tiyasatkulkovit, Wacharaporn

AU - Malaivijitnond, Suchinda

AU - Charoenphandhu, Narattaphol

AU - Havill, Lorena M.

AU - Ford, Allen L.

AU - Vandeberg, John L.

PY - 2014/10/15

Y1 - 2014/10/15

N2 - Phytoestrogen-rich Pueraria mirifica (PM) tuberous extract is a promising candidate for the development of anti-osteoporosis drugs for postmenopausal women, but its action has never been validated in humans or in non-human primates, which are more closely related to humans than rodents. In vitro study of non-human primate osteoblasts is thus fundamental to prepare for in vivo studies of phytoestrogen effects on primate bone. This study aimed to establish a culture system of baboon primary osteoblasts and to investigate the effects of PM extract and its phytoestrogens on these cells. Primary osteoblasts from adult baboon fibulae exhibited osteoblast characteristics in regard to proliferation, differentiation, mineralization, and estrogen receptor expression. They responded to 17β-estradiol by increased proliferation rate and mRNA levels of alkaline phosphatase (ALP), type I collagen, and osteocalcin. After being exposed for 48 h to 100 μg/ml PM extract, 1000 nM genistein, or 1000 nM puerarin, primary baboon osteoblasts markedly increased the rate of proliferation and mRNA levels of ALP and type I collagen without changes in Runx2, osterix, or osteocalcin expression. PM extract, genistein, and puerarin also decreased the RANKL/OPG ratio, suggesting that they could decrease osteoclast-mediated bone resorption. However, neither PM extract nor its phytoestrogens altered calcium deposition in osteoblast culture. In conclusion, we have established baboon primary osteoblast culture, which is a new tool for bone research and drug discovery. Furthermore, the present results provide substantial support for the potential of PM extract and its phytoestrogens to be developed as therapeutic agents against bone fragility.

AB - Phytoestrogen-rich Pueraria mirifica (PM) tuberous extract is a promising candidate for the development of anti-osteoporosis drugs for postmenopausal women, but its action has never been validated in humans or in non-human primates, which are more closely related to humans than rodents. In vitro study of non-human primate osteoblasts is thus fundamental to prepare for in vivo studies of phytoestrogen effects on primate bone. This study aimed to establish a culture system of baboon primary osteoblasts and to investigate the effects of PM extract and its phytoestrogens on these cells. Primary osteoblasts from adult baboon fibulae exhibited osteoblast characteristics in regard to proliferation, differentiation, mineralization, and estrogen receptor expression. They responded to 17β-estradiol by increased proliferation rate and mRNA levels of alkaline phosphatase (ALP), type I collagen, and osteocalcin. After being exposed for 48 h to 100 μg/ml PM extract, 1000 nM genistein, or 1000 nM puerarin, primary baboon osteoblasts markedly increased the rate of proliferation and mRNA levels of ALP and type I collagen without changes in Runx2, osterix, or osteocalcin expression. PM extract, genistein, and puerarin also decreased the RANKL/OPG ratio, suggesting that they could decrease osteoclast-mediated bone resorption. However, neither PM extract nor its phytoestrogens altered calcium deposition in osteoblast culture. In conclusion, we have established baboon primary osteoblast culture, which is a new tool for bone research and drug discovery. Furthermore, the present results provide substantial support for the potential of PM extract and its phytoestrogens to be developed as therapeutic agents against bone fragility.

KW - Baboon

KW - Genistein

KW - Osteoblasts

KW - Osteoporosis

KW - Pueraria mirifica

KW - Puerarin

UR - http://www.scopus.com/inward/record.url?scp=84907212868&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84907212868&partnerID=8YFLogxK

U2 - 10.1016/j.phymed.2014.06.019

DO - 10.1016/j.phymed.2014.06.019

M3 - Article

C2 - 25442257

AN - SCOPUS:84907212868

VL - 21

SP - 1498

EP - 1503

JO - Phytomedicine

JF - Phytomedicine

SN - 0944-7113

IS - 12

ER -