Public Health

BACKGROUND: The American Heart Association has identified eight metrics for improving heart and brain health, including sleep health. Recent studies highlight the strong link between sleep health, gut microbiome, and diseases like Alzheimer's. While sleep deprivation is known to affect gut microbiome and brain health, the specific impact of sleep health on gut microbiome and cognitive disorders remains largely unexplored. METHOD: We analyzed stool samples and sleep metrics from 781 participants (mean age 54.9, 57.1% female) in the Framingham Heart Study to examine the effect of sleep on gut microbiome composition and cognitive performance. Using the V4 region of the 16S rRNA and Lefse analysis, we identified microbiome profiles related to sleep health. ANOVA assessed the sleep-cognitive performance relationship, while multivariable and differential abundance analyses explored the microbiome's link to cognitive function, controlling for age, sex, and education. RESULT: Differences in bacterial diversity were observed between low, moderate, and high groups. Lefse analysis showed higher levels of aldenense, bolteae, symbiosium, and lavalense in the low group, while Butyrivibrio, putredinis, and Dorea were less abundant. ANOVA indicated a significant correlation between global cognitive scores and sleep metrics (p = 0.0024). Positive correlations were found between cognitive scores and Pseudobutyrivibrio and Ruminococcus, while negative correlations were observed with Barnesiella and Clostridium. At the species level, xylanivorans and lactaris were positively correlated, whereas boltea, callidus, and intestinihominis were negatively correlated with cognitive scores. CONCLUSION: Our findings showed that individuals with good sleep scores had higher cognitive performance, while those with lower sleep scores had lower cognitive performance. The results also indicated an association between gut microbiome and sleep metric as well as between gut microbiome and cognitive performance. Finally, our work revealed that the taxa Clostridium and bolteae exhibited association with both sleep metric and cognitive performance. Further studies should be conducted to understand the effects of sleep metric on the relationship between gut microbiome and the risk of developing Alzheimer's Disease and Related Dementias (ADRD).