Proximity of 5.8 S RNA-binding proteins and A-site proteins in yeast ribosomes inferred from cross-linking

J. C. Lee, R. R. Traut

Research output: Contribution to journalArticle

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Abstract

In order to probe the spatial arrangement of proteins within the 5.8 S rRNA domain of the intact ribosomal subunit of Saccharomyces cerevisiae, 60 S ribosomal subunits were treated with 2-iminothiolane. Proteins were extracted from the cross-linked ribosomes, fractionated by Sephadex G-150 column chromatography, and analyzed by diagonal polyacrylamide-sodium dodecyl sulfate gel electrophoresis. Constituent proteins of cross-linked pairs were identified by two-dimensional polyacrylamide gel electrophoresis. Nine cross-links involving five of the 11 5.8 S rRNA-binding proteins were analyzed. A model showing the network of cross-links is presented. Several of the 5.8 S rRNA-binding proteins are in sufficiently close proximity of the ribosomal A-site proteins to be cross-linked.

Original languageEnglish (US)
Pages (from-to)9971-9974
Number of pages4
JournalJournal of Biological Chemistry
Volume259
Issue number16
Publication statusPublished - Jan 1 1984

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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