Proximal region of the gene encoding cytadherence-related protein permits molecular typing of Mycoplasma genitalium clinical strains by PCR-restriction fragment length polymorphism

Oxana Musatovova, Caleb Herrera, Joel B. Baseman

Research output: Contribution to journalArticle

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Abstract

Restriction fragment length polymorphism (RFLP) analysis of the PCR-amplified proximal region of the gene encoding cytadherence accessory protein P110 (MG192) revealed DNA sequence divergences among 54 Mycoplasma genitalium clinical strains isolated from the genitourinary tracts of women attending a sexually transmitted disease-related health clinic, plus one from the respiratory tract and one from synovial fluid. Seven of 56 (12.5%) strains exhibited RFLPs following digestion of the proximal region with restriction endonuclease MboI or RsaI, or both. No sequence variability was detected in the distal portion of the gene.

Original languageEnglish (US)
Pages (from-to)598-603
Number of pages6
JournalJournal of clinical microbiology
Volume44
Issue number2
DOIs
Publication statusPublished - Feb 1 2006

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ASJC Scopus subject areas

  • Microbiology (medical)

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