Protocol for hybrid structured-light three-dimensional scanning and modeling of human postmortem brains

Morgan Mekale Smith; Elizabeth Ochoa; Savannah Barannikov; Mallory Keating; Mohamad Habes; Margaret E. Flanagan; Sudha Seshadri; Kevin F. Bieniek

doi:10.1016/j.xpro.2024.103246

Protocol for hybrid structured-light three-dimensional scanning and modeling of human postmortem brains

Morgan Mekale Smith, Elizabeth Ochoa, Savannah Barannikov, Mallory Keating, Mohamad Habes, Margaret E. Flanagan, Sudha Seshadri, Kevin F. Bieniek

Research output: Contribution to journal › Article › peer-review

Abstract

Despite numerous neuropathological criteria for the evaluation of microscopic tissue slides, there are no systemic standards for sectioning postmortem human brain tissue. Here, we present a protocol for postmortem brain hybrid structured-light scanning to facilitate 3D printing of sectioning matrices. We describe steps for tissue preparation, 3D scanning and printing, and matrix-guided sectioning. This protocol can be used for streamlining tissue histopathology as well as in brain morphology volumetric analyses and applications in medical education. For complete details on the use and execution of this protocol, please refer to Barannikov et al.¹

Original language	English (US)
Article number	103246
Journal	STAR Protocols
Volume	5
Issue number	3
DOIs	https://doi.org/10.1016/j.xpro.2024.103246
State	Published - Sep 20 2024

Keywords

Biotechnology and bioengineering
Health Sciences
Neuroscience
Systems biology

ASJC Scopus subject areas

General Neuroscience
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology

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10.1016/j.xpro.2024.103246

Cite this

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abstract = "Despite numerous neuropathological criteria for the evaluation of microscopic tissue slides, there are no systemic standards for sectioning postmortem human brain tissue. Here, we present a protocol for postmortem brain hybrid structured-light scanning to facilitate 3D printing of sectioning matrices. We describe steps for tissue preparation, 3D scanning and printing, and matrix-guided sectioning. This protocol can be used for streamlining tissue histopathology as well as in brain morphology volumetric analyses and applications in medical education. For complete details on the use and execution of this protocol, please refer to Barannikov et al.1",

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AU - Smith, Morgan Mekale

AU - Ochoa, Elizabeth

AU - Barannikov, Savannah

AU - Keating, Mallory

AU - Habes, Mohamad

AU - Flanagan, Margaret E.

AU - Seshadri, Sudha

AU - Bieniek, Kevin F.

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AB - Despite numerous neuropathological criteria for the evaluation of microscopic tissue slides, there are no systemic standards for sectioning postmortem human brain tissue. Here, we present a protocol for postmortem brain hybrid structured-light scanning to facilitate 3D printing of sectioning matrices. We describe steps for tissue preparation, 3D scanning and printing, and matrix-guided sectioning. This protocol can be used for streamlining tissue histopathology as well as in brain morphology volumetric analyses and applications in medical education. For complete details on the use and execution of this protocol, please refer to Barannikov et al.1

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Protocol for hybrid structured-light three-dimensional scanning and modeling of human postmortem brains

Abstract

Keywords

ASJC Scopus subject areas

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