Protocol for assessing phagocytosis activity in cultured primary murine microglia

Elsie Layman, Jennifer Michelle Parrott, Hye Young Lee

Research output: Contribution to journalArticlepeer-review

Abstract

In this protocol, we describe steps to assess inflammation-induced cell response in cultured primary murine microglia through the analysis of fluorescent bead phagocytosis. We detail primary murine mixed glial cell culture preparation followed by microglia-specific isolation. Further, we describe treatment with lipopolysaccharide (LPS) to induce phagocytosis of fluorescent beads, followed by quantitative analysis using fluorescent imaging and Fiji – ImageJ software. For complete details on the use and execution of this protocol, please refer to Parrott et al.1

Original languageEnglish (US)
Article number101881
JournalSTAR Protocols
Volume3
Issue number4
DOIs
StatePublished - Dec 16 2022
Externally publishedYes

Keywords

  • Cell Biology
  • Cell culture
  • Cell-based Assays
  • Microscopy
  • Neuroscience

ASJC Scopus subject areas

  • General Immunology and Microbiology
  • General Biochemistry, Genetics and Molecular Biology
  • General Neuroscience

Fingerprint

Dive into the research topics of 'Protocol for assessing phagocytosis activity in cultured primary murine microglia'. Together they form a unique fingerprint.

Cite this