Protocol for assessing phagocytosis activity in cultured primary murine microglia

Elsie Layman, Jennifer Michelle Parrott, Hye Young Lee

Research output: Contribution to journalArticlepeer-review


In this protocol, we describe steps to assess inflammation-induced cell response in cultured primary murine microglia through the analysis of fluorescent bead phagocytosis. We detail primary murine mixed glial cell culture preparation followed by microglia-specific isolation. Further, we describe treatment with lipopolysaccharide (LPS) to induce phagocytosis of fluorescent beads, followed by quantitative analysis using fluorescent imaging and Fiji – ImageJ software. For complete details on the use and execution of this protocol, please refer to Parrott et al.1

Original languageEnglish (US)
Article number101881
JournalSTAR Protocols
Issue number4
StatePublished - Dec 16 2022
Externally publishedYes


  • Cell Biology
  • Cell culture
  • Cell-based Assays
  • Microscopy
  • Neuroscience

ASJC Scopus subject areas

  • General Immunology and Microbiology
  • General Biochemistry, Genetics and Molecular Biology
  • General Neuroscience


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