Abstract
Urinary calculi composed of struvite harbor urease-producing bacteria within the stone. The photothermal mechanism of holmium:YAG lithotripsy is uniquely different than other lithotripsy devices. We postulated that bacterial viability of struvite calculi would be less for calculi fragmented with holmium:YAG irradiation compared to other lithotripsy devices. Human calculi of known struvite composition (greater than 90% magnesium ammonium phosphate hexahydrate) were incubated with Proteus mirabilis. Calculi were fragmented with no lithotripsy (controls), or shock wave, intracorporeal ultrasonic, electrohydraulic, pneumatic, holmium:YAG or pulsed dye laser lithotripsy. After lithotripsy, stone fragments were sonicated and specimens were serially plated for 48 hours at 38C. Bacterial counts and the rate of bacterial sterilization were compared. Median bacterial counts (colony forming units per ml) were 8×10 6 in controls and 3×10 6 in shock wave, 3×10 7 in ultrasonic, 4×10 5 in electrohydraulic, 8×10 6 in pneumatic, 5×10 4 in holmium:YAG and 1×10 6 in pulsed dye laser lithotripsy, p<0.001. The rate of bacterial sterilization was 50% for holmium:YAG lithotripsy treated stones versus 0% for each of the other cohorts, p<0.01. P. mirabills viability is less after holmium:YAG irradiation compared to other lithotripsy devices.
| Original language | English (US) |
|---|---|
| Title of host publication | Proceedings of SPIE - The International Society for Optical Engineering |
| Publisher | Society of Photo-Optical Instrumentation Engineers |
| Pages | 240-244 |
| Number of pages | 5 |
| Volume | 3907 |
| State | Published - 2000 |
| Externally published | Yes |
| Event | Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems X - San Jose, CA, USA Duration: Jan 22 2000 → Jan 25 2000 |
Other
| Other | Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems X |
|---|---|
| City | San Jose, CA, USA |
| Period | 1/22/00 → 1/25/00 |
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ASJC Scopus subject areas
- Electrical and Electronic Engineering
- Condensed Matter Physics
Cite this
Proteus mirabilis viability after lithotripsy of struvite calculi. / Prabakharan, Sabitha; Teichman, Joel M H; Spore, Scott S.; Sabanegh, Edmund; Glickman, Randolph D; McLean, Robert J C.
Proceedings of SPIE - The International Society for Optical Engineering. Vol. 3907 Society of Photo-Optical Instrumentation Engineers, 2000. p. 240-244.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Proteus mirabilis viability after lithotripsy of struvite calculi
AU - Prabakharan, Sabitha
AU - Teichman, Joel M H
AU - Spore, Scott S.
AU - Sabanegh, Edmund
AU - Glickman, Randolph D
AU - McLean, Robert J C
PY - 2000
Y1 - 2000
N2 - Urinary calculi composed of struvite harbor urease-producing bacteria within the stone. The photothermal mechanism of holmium:YAG lithotripsy is uniquely different than other lithotripsy devices. We postulated that bacterial viability of struvite calculi would be less for calculi fragmented with holmium:YAG irradiation compared to other lithotripsy devices. Human calculi of known struvite composition (greater than 90% magnesium ammonium phosphate hexahydrate) were incubated with Proteus mirabilis. Calculi were fragmented with no lithotripsy (controls), or shock wave, intracorporeal ultrasonic, electrohydraulic, pneumatic, holmium:YAG or pulsed dye laser lithotripsy. After lithotripsy, stone fragments were sonicated and specimens were serially plated for 48 hours at 38C. Bacterial counts and the rate of bacterial sterilization were compared. Median bacterial counts (colony forming units per ml) were 8×10 6 in controls and 3×10 6 in shock wave, 3×10 7 in ultrasonic, 4×10 5 in electrohydraulic, 8×10 6 in pneumatic, 5×10 4 in holmium:YAG and 1×10 6 in pulsed dye laser lithotripsy, p<0.001. The rate of bacterial sterilization was 50% for holmium:YAG lithotripsy treated stones versus 0% for each of the other cohorts, p<0.01. P. mirabills viability is less after holmium:YAG irradiation compared to other lithotripsy devices.
AB - Urinary calculi composed of struvite harbor urease-producing bacteria within the stone. The photothermal mechanism of holmium:YAG lithotripsy is uniquely different than other lithotripsy devices. We postulated that bacterial viability of struvite calculi would be less for calculi fragmented with holmium:YAG irradiation compared to other lithotripsy devices. Human calculi of known struvite composition (greater than 90% magnesium ammonium phosphate hexahydrate) were incubated with Proteus mirabilis. Calculi were fragmented with no lithotripsy (controls), or shock wave, intracorporeal ultrasonic, electrohydraulic, pneumatic, holmium:YAG or pulsed dye laser lithotripsy. After lithotripsy, stone fragments were sonicated and specimens were serially plated for 48 hours at 38C. Bacterial counts and the rate of bacterial sterilization were compared. Median bacterial counts (colony forming units per ml) were 8×10 6 in controls and 3×10 6 in shock wave, 3×10 7 in ultrasonic, 4×10 5 in electrohydraulic, 8×10 6 in pneumatic, 5×10 4 in holmium:YAG and 1×10 6 in pulsed dye laser lithotripsy, p<0.001. The rate of bacterial sterilization was 50% for holmium:YAG lithotripsy treated stones versus 0% for each of the other cohorts, p<0.01. P. mirabills viability is less after holmium:YAG irradiation compared to other lithotripsy devices.
UR - http://www.scopus.com/inward/record.url?scp=0033707272&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033707272&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:0033707272
VL - 3907
SP - 240
EP - 244
BT - Proceedings of SPIE - The International Society for Optical Engineering
PB - Society of Photo-Optical Instrumentation Engineers
ER -