Proteus mirabilis viability after lithotripsy of struvite calculi

Sabitha Prabakharan, Joel M H Teichman, Scott S. Spore, Edmund Sabanegh, Randolph D Glickman, Robert J C McLean

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Urinary calculi composed of struvite harbor urease-producing bacteria within the stone. The photothermal mechanism of holmium:YAG lithotripsy is uniquely different than other lithotripsy devices. We postulated that bacterial viability of struvite calculi would be less for calculi fragmented with holmium:YAG irradiation compared to other lithotripsy devices. Human calculi of known struvite composition (greater than 90% magnesium ammonium phosphate hexahydrate) were incubated with Proteus mirabilis. Calculi were fragmented with no lithotripsy (controls), or shock wave, intracorporeal ultrasonic, electrohydraulic, pneumatic, holmium:YAG or pulsed dye laser lithotripsy. After lithotripsy, stone fragments were sonicated and specimens were serially plated for 48 hours at 38C. Bacterial counts and the rate of bacterial sterilization were compared. Median bacterial counts (colony forming units per ml) were 8×10 6 in controls and 3×10 6 in shock wave, 3×10 7 in ultrasonic, 4×10 5 in electrohydraulic, 8×10 6 in pneumatic, 5×10 4 in holmium:YAG and 1×10 6 in pulsed dye laser lithotripsy, p<0.001. The rate of bacterial sterilization was 50% for holmium:YAG lithotripsy treated stones versus 0% for each of the other cohorts, p<0.01. P. mirabills viability is less after holmium:YAG irradiation compared to other lithotripsy devices.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherSociety of Photo-Optical Instrumentation Engineers
Pages240-244
Number of pages5
Volume3907
StatePublished - 2000
Externally publishedYes
EventLasers in Surgery: Advanced Characterization, Therapeutics, and Systems X - San Jose, CA, USA
Duration: Jan 22 2000Jan 25 2000

Other

OtherLasers in Surgery: Advanced Characterization, Therapeutics, and Systems X
CitySan Jose, CA, USA
Period1/22/001/25/00

Fingerprint

Proteus
calculi
Holmium
holmium
viability
yttrium-aluminum garnet
Dye lasers
pneumatics
rocks
Pulsed lasers
dye lasers
Shock waves
Pneumatics
shock waves
pulsed lasers
ultrasonics
Ultrasonics
Irradiation
ammonium phosphates
irradiation

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Prabakharan, S., Teichman, J. M. H., Spore, S. S., Sabanegh, E., Glickman, R. D., & McLean, R. J. C. (2000). Proteus mirabilis viability after lithotripsy of struvite calculi. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 3907, pp. 240-244). Society of Photo-Optical Instrumentation Engineers.

Proteus mirabilis viability after lithotripsy of struvite calculi. / Prabakharan, Sabitha; Teichman, Joel M H; Spore, Scott S.; Sabanegh, Edmund; Glickman, Randolph D; McLean, Robert J C.

Proceedings of SPIE - The International Society for Optical Engineering. Vol. 3907 Society of Photo-Optical Instrumentation Engineers, 2000. p. 240-244.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Prabakharan, S, Teichman, JMH, Spore, SS, Sabanegh, E, Glickman, RD & McLean, RJC 2000, Proteus mirabilis viability after lithotripsy of struvite calculi. in Proceedings of SPIE - The International Society for Optical Engineering. vol. 3907, Society of Photo-Optical Instrumentation Engineers, pp. 240-244, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems X, San Jose, CA, USA, 1/22/00.
Prabakharan S, Teichman JMH, Spore SS, Sabanegh E, Glickman RD, McLean RJC. Proteus mirabilis viability after lithotripsy of struvite calculi. In Proceedings of SPIE - The International Society for Optical Engineering. Vol. 3907. Society of Photo-Optical Instrumentation Engineers. 2000. p. 240-244
Prabakharan, Sabitha ; Teichman, Joel M H ; Spore, Scott S. ; Sabanegh, Edmund ; Glickman, Randolph D ; McLean, Robert J C. / Proteus mirabilis viability after lithotripsy of struvite calculi. Proceedings of SPIE - The International Society for Optical Engineering. Vol. 3907 Society of Photo-Optical Instrumentation Engineers, 2000. pp. 240-244
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abstract = "Urinary calculi composed of struvite harbor urease-producing bacteria within the stone. The photothermal mechanism of holmium:YAG lithotripsy is uniquely different than other lithotripsy devices. We postulated that bacterial viability of struvite calculi would be less for calculi fragmented with holmium:YAG irradiation compared to other lithotripsy devices. Human calculi of known struvite composition (greater than 90{\%} magnesium ammonium phosphate hexahydrate) were incubated with Proteus mirabilis. Calculi were fragmented with no lithotripsy (controls), or shock wave, intracorporeal ultrasonic, electrohydraulic, pneumatic, holmium:YAG or pulsed dye laser lithotripsy. After lithotripsy, stone fragments were sonicated and specimens were serially plated for 48 hours at 38C. Bacterial counts and the rate of bacterial sterilization were compared. Median bacterial counts (colony forming units per ml) were 8×10 6 in controls and 3×10 6 in shock wave, 3×10 7 in ultrasonic, 4×10 5 in electrohydraulic, 8×10 6 in pneumatic, 5×10 4 in holmium:YAG and 1×10 6 in pulsed dye laser lithotripsy, p<0.001. The rate of bacterial sterilization was 50{\%} for holmium:YAG lithotripsy treated stones versus 0{\%} for each of the other cohorts, p<0.01. P. mirabills viability is less after holmium:YAG irradiation compared to other lithotripsy devices.",
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