Proteus mirabilis viability after lithotripsy of struvite calculi

Sabitha Prabakharan, Joel M.H. Teichman, Scott S. Spore, Edmund Sabanegh, Randolph D. Glickman, Robert J.C. McLean

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Purpose: We tested the hypotheses that Proteus mirabilis viability of struvite calculi differs after exposure to different lithotripsy modalities and that the photothermal mechanism of holmium:YAG lithotripsy is antibacterial. Materials and Methods: Human calculi of known struvite composition (greater than 90% magnesium ammonium phosphate hexohydrate) were incubated with P. mirabilis. Calculi were randomly distributed and fragmented with no lithotripsy (controls), or shock wave, intracorporeal ultrasonic, electrohydraulic, pneumatic, holmium:YAG or pulsed dye laser lithotripsy. After lithotripsy fragments were sonicated and specimens were serially plated for 48 hours at 38C. Bacterial counts and the rate of bacterial sterilization were compared. Results: Median bacterial counts (colony-forming units per ml.) were 8 x 106 in controls and 3 x 106 in shock wave, 3 x 107 in ultrasonic, 4 x 105 in electrohydraulic, 8 x 106 in pneumatic, 5 x 104 in holmium:YAG and 1 x 106 in pulsed dye laser lithotripsy cases (p <0.001). The rate of bacterial sterilization was 50% for holmium:YAG lithotripsy treated stones versus 0% for each of the other cohorts (p <0.01). Conclusions: P. mirabilis viability varies among lithotrites. The photothermal mechanism of holmium:YAG lithotripsy is antibacterial.

Original languageEnglish (US)
Pages (from-to)1666-1669
Number of pages4
JournalJournal of Urology
Volume162
Issue number5
DOIs
StatePublished - Nov 1999

Keywords

  • Lithotripsy
  • Proteus mirabilis
  • Urinary calculi
  • Urinary tract infections

ASJC Scopus subject areas

  • Urology

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    Prabakharan, S., Teichman, J. M. H., Spore, S. S., Sabanegh, E., Glickman, R. D., & McLean, R. J. C. (1999). Proteus mirabilis viability after lithotripsy of struvite calculi. Journal of Urology, 162(5), 1666-1669. https://doi.org/10.1016/S0022-5347(05)68192-X