Proteomic analysis of the left ventricle post-myocardial infarction to identify in vivo candidate matrix metalloproteinase substrates

Andriy Yabluchanskiy, Yaojun Li, Lisandra E. De Castro Brás, Kevin Hakala, Susan T. Weintraub, Merry L. Lindsey

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

Left ventricular remodeling post-myocardial infarction (MI) involves a multitude of mechanisms that regulate the repair response. Matrix metalloproteinases (MMPs) are a major family of proteolytic enzymes that coordinate extracellular matrix turnover. MMP-7 or MMP-9 deletion attenuate adverse remodeling post-MI, but the mechanisms have not been fully clarified. Both MMP-7 and MMP-9 have a large number of known in vitro substrates, but in vivo substrates for these two MMPs in the myocardial infarction setting are incompletely identified. Advances in proteomic techniques have enabled comprehensive profiling of protein expression in cells and tissue. In this chapter, we describe a protocol for the proteomic analysis of in vivo candidate MMP substrates in the post-MI left ventricle using two-dimensional electrophoresis, liquid chromatography coupled with tandem mass spectrometry, and immunoblotting.

Original languageEnglish (US)
Title of host publicationCell-Cell Interactions
Subtitle of host publicationMethods and Protocols
PublisherHumana Press
Pages185-199
Number of pages15
ISBN (Print)9781627036030
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1066
ISSN (Print)1064-3745

Keywords

  • Cardiac remodeling
  • Extracellular matrix
  • Matrix metalloproteinase
  • Mice
  • Myocardial infarction
  • Proteomics
  • Substrates

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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