Abstract
Susceptibility of membrane proteins to digestion with chymotrypsin was compared in various age fractions of bovine erythrocytes by SDS-polyacrylamide gel electrophoresis. The extent of protein digestion was found to be higher in membranes of older erythrocytes, as judged from the disappearance of original protein bands and accumulation of digestion products. These results are consistent with the hypothesis of a proteolytic generation of a "senescent cel antigen" in red blood cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 109-121 |
| Number of pages | 13 |
| Journal | Mechanisms of Ageing and Development |
| Volume | 35 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1986 |
| Externally published | Yes |
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Keywords
- Erythrocyte aging
- Membrane proteins
- Proteolytic susceptibility
ASJC Scopus subject areas
- Aging
- Biochemistry
- Developmental Biology
- Developmental Neuroscience
Cite this
Proteolytic susceptibility of membrane proteins during erythrocyte aging. / Gaczynska, Maria E; Rosin, Janusz; Soszyński, Mirosław; Bartosz, Grzegorz.
In: Mechanisms of Ageing and Development, Vol. 35, No. 2, 1986, p. 109-121.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Proteolytic susceptibility of membrane proteins during erythrocyte aging
AU - Gaczynska, Maria E
AU - Rosin, Janusz
AU - Soszyński, Mirosław
AU - Bartosz, Grzegorz
PY - 1986
Y1 - 1986
N2 - Susceptibility of membrane proteins to digestion with chymotrypsin was compared in various age fractions of bovine erythrocytes by SDS-polyacrylamide gel electrophoresis. The extent of protein digestion was found to be higher in membranes of older erythrocytes, as judged from the disappearance of original protein bands and accumulation of digestion products. These results are consistent with the hypothesis of a proteolytic generation of a "senescent cel antigen" in red blood cells.
AB - Susceptibility of membrane proteins to digestion with chymotrypsin was compared in various age fractions of bovine erythrocytes by SDS-polyacrylamide gel electrophoresis. The extent of protein digestion was found to be higher in membranes of older erythrocytes, as judged from the disappearance of original protein bands and accumulation of digestion products. These results are consistent with the hypothesis of a proteolytic generation of a "senescent cel antigen" in red blood cells.
KW - Erythrocyte aging
KW - Membrane proteins
KW - Proteolytic susceptibility
UR - http://www.scopus.com/inward/record.url?scp=0022478589&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022478589&partnerID=8YFLogxK
U2 - 10.1016/0047-6374(86)90002-3
DO - 10.1016/0047-6374(86)90002-3
M3 - Article
C2 - 3736134
AN - SCOPUS:0022478589
VL - 35
SP - 109
EP - 121
JO - Mechanisms of Ageing and Development
JF - Mechanisms of Ageing and Development
SN - 0047-6374
IS - 2
ER -