Proteolytic susceptibility of membrane proteins during erythrocyte aging

Maria E Gaczynska, Janusz Rosin, Mirosław Soszyński, Grzegorz Bartosz

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Susceptibility of membrane proteins to digestion with chymotrypsin was compared in various age fractions of bovine erythrocytes by SDS-polyacrylamide gel electrophoresis. The extent of protein digestion was found to be higher in membranes of older erythrocytes, as judged from the disappearance of original protein bands and accumulation of digestion products. These results are consistent with the hypothesis of a proteolytic generation of a "senescent cel antigen" in red blood cells.

Original languageEnglish (US)
Pages (from-to)109-121
Number of pages13
JournalMechanisms of Ageing and Development
Volume35
Issue number2
DOIs
StatePublished - 1986
Externally publishedYes

Fingerprint

Erythrocyte Aging
Proteolysis
Membrane Proteins
Aging of materials
Erythrocytes
Chymotrypsin
Erythrocyte Membrane
Electrophoresis
Polyacrylamide Gel Electrophoresis
Digestion
Proteins
Blood
Cells
Membranes
Antigens

Keywords

  • Erythrocyte aging
  • Membrane proteins
  • Proteolytic susceptibility

ASJC Scopus subject areas

  • Aging
  • Biochemistry
  • Developmental Biology
  • Developmental Neuroscience

Cite this

Proteolytic susceptibility of membrane proteins during erythrocyte aging. / Gaczynska, Maria E; Rosin, Janusz; Soszyński, Mirosław; Bartosz, Grzegorz.

In: Mechanisms of Ageing and Development, Vol. 35, No. 2, 1986, p. 109-121.

Research output: Contribution to journalArticle

Gaczynska, Maria E ; Rosin, Janusz ; Soszyński, Mirosław ; Bartosz, Grzegorz. / Proteolytic susceptibility of membrane proteins during erythrocyte aging. In: Mechanisms of Ageing and Development. 1986 ; Vol. 35, No. 2. pp. 109-121.
@article{6e313889178249409ad907789506a37c,
title = "Proteolytic susceptibility of membrane proteins during erythrocyte aging",
abstract = "Susceptibility of membrane proteins to digestion with chymotrypsin was compared in various age fractions of bovine erythrocytes by SDS-polyacrylamide gel electrophoresis. The extent of protein digestion was found to be higher in membranes of older erythrocytes, as judged from the disappearance of original protein bands and accumulation of digestion products. These results are consistent with the hypothesis of a proteolytic generation of a {"}senescent cel antigen{"} in red blood cells.",
keywords = "Erythrocyte aging, Membrane proteins, Proteolytic susceptibility",
author = "Gaczynska, {Maria E} and Janusz Rosin and Mirosław Soszyński and Grzegorz Bartosz",
year = "1986",
doi = "10.1016/0047-6374(86)90002-3",
language = "English (US)",
volume = "35",
pages = "109--121",
journal = "Mechanisms of Ageing and Development",
issn = "0047-6374",
publisher = "Elsevier Ireland Ltd",
number = "2",

}

TY - JOUR

T1 - Proteolytic susceptibility of membrane proteins during erythrocyte aging

AU - Gaczynska, Maria E

AU - Rosin, Janusz

AU - Soszyński, Mirosław

AU - Bartosz, Grzegorz

PY - 1986

Y1 - 1986

N2 - Susceptibility of membrane proteins to digestion with chymotrypsin was compared in various age fractions of bovine erythrocytes by SDS-polyacrylamide gel electrophoresis. The extent of protein digestion was found to be higher in membranes of older erythrocytes, as judged from the disappearance of original protein bands and accumulation of digestion products. These results are consistent with the hypothesis of a proteolytic generation of a "senescent cel antigen" in red blood cells.

AB - Susceptibility of membrane proteins to digestion with chymotrypsin was compared in various age fractions of bovine erythrocytes by SDS-polyacrylamide gel electrophoresis. The extent of protein digestion was found to be higher in membranes of older erythrocytes, as judged from the disappearance of original protein bands and accumulation of digestion products. These results are consistent with the hypothesis of a proteolytic generation of a "senescent cel antigen" in red blood cells.

KW - Erythrocyte aging

KW - Membrane proteins

KW - Proteolytic susceptibility

UR - http://www.scopus.com/inward/record.url?scp=0022478589&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022478589&partnerID=8YFLogxK

U2 - 10.1016/0047-6374(86)90002-3

DO - 10.1016/0047-6374(86)90002-3

M3 - Article

C2 - 3736134

AN - SCOPUS:0022478589

VL - 35

SP - 109

EP - 121

JO - Mechanisms of Ageing and Development

JF - Mechanisms of Ageing and Development

SN - 0047-6374

IS - 2

ER -