Proteolytic susceptibility of membrane proteins during erythrocyte aging

Maria Gaczyńska; Janusz Rosin; Mirosław Soszyński; Grzegorz Bartosz

doi:10.1016/0047-6374(86)90002-3

Proteolytic susceptibility of membrane proteins during erythrocyte aging

Maria Gaczyńska, Janusz Rosin, Mirosław Soszyński, Grzegorz Bartosz

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Susceptibility of membrane proteins to digestion with chymotrypsin was compared in various age fractions of bovine erythrocytes by SDS-polyacrylamide gel electrophoresis. The extent of protein digestion was found to be higher in membranes of older erythrocytes, as judged from the disappearance of original protein bands and accumulation of digestion products. These results are consistent with the hypothesis of a proteolytic generation of a "senescent cel antigen" in red blood cells.

Original language	English (US)
Pages (from-to)	109-121
Number of pages	13
Journal	Mechanisms of Ageing and Development
Volume	35
Issue number	2
DOIs	https://doi.org/10.1016/0047-6374(86)90002-3
State	Published - Jul 1986
Externally published	Yes

Keywords

Erythrocyte aging
Membrane proteins
Proteolytic susceptibility

ASJC Scopus subject areas

Aging
Developmental Biology

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title = "Proteolytic susceptibility of membrane proteins during erythrocyte aging",

abstract = "Susceptibility of membrane proteins to digestion with chymotrypsin was compared in various age fractions of bovine erythrocytes by SDS-polyacrylamide gel electrophoresis. The extent of protein digestion was found to be higher in membranes of older erythrocytes, as judged from the disappearance of original protein bands and accumulation of digestion products. These results are consistent with the hypothesis of a proteolytic generation of a {"}senescent cel antigen{"} in red blood cells.",

keywords = "Erythrocyte aging, Membrane proteins, Proteolytic susceptibility",

author = "Maria Gaczy{\'n}ska and Janusz Rosin and Miros{\l}aw Soszy{\'n}ski and Grzegorz Bartosz",

year = "1986",

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T1 - Proteolytic susceptibility of membrane proteins during erythrocyte aging

AU - Gaczyńska, Maria

AU - Rosin, Janusz

AU - Soszyński, Mirosław

AU - Bartosz, Grzegorz

PY - 1986/7

Y1 - 1986/7

N2 - Susceptibility of membrane proteins to digestion with chymotrypsin was compared in various age fractions of bovine erythrocytes by SDS-polyacrylamide gel electrophoresis. The extent of protein digestion was found to be higher in membranes of older erythrocytes, as judged from the disappearance of original protein bands and accumulation of digestion products. These results are consistent with the hypothesis of a proteolytic generation of a "senescent cel antigen" in red blood cells.

AB - Susceptibility of membrane proteins to digestion with chymotrypsin was compared in various age fractions of bovine erythrocytes by SDS-polyacrylamide gel electrophoresis. The extent of protein digestion was found to be higher in membranes of older erythrocytes, as judged from the disappearance of original protein bands and accumulation of digestion products. These results are consistent with the hypothesis of a proteolytic generation of a "senescent cel antigen" in red blood cells.

KW - Erythrocyte aging

KW - Membrane proteins

KW - Proteolytic susceptibility

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