Proteinase detection, DNA typing and antimycotic susceptibility of Candida isolates from Colombian women with vulvovaginal candidiasis

Nora Cardona-Castro, Sanjay G. Revankar, Patricia Ortiz, Claudia Cuervo, William R. Kirkpatrick, Robert K. McAtee, Thomas F Patterson

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Forty non-pregnant Colombian women (ages 18 - 45) with vulvovaginal candidiasis diagnosis (VVC) were enrolled in a blinded study to compare the efficacy of Itraconazole (ITRA) 400 mg vs. Fluconazole (FLU) 150 mg. Sexual partners received similar therapy. Proteinase detection by the Staib method and minimal inhibitory concentration (MIC) for FLU and ITRA by Etest method were performed in all Candida isolates. Patients were followed one year to determine clinical evolution and recurrence of VVC (RVVC). The strain identity of the RVVC isolates was determined by contour-clamped homogeneous electric field (CHEF) gel electrophoresis karyotyping and restriction fragment length polymorphism (RFLP). Thirty patients (75%) had one or two episodes of VVC/year, 83% of these were due to Candida albicans, while ten patients (25%) developed RVVC (three or more episodes/year); seven of them were treated with FLU. Non-C. albicans Candida species were detected in five of 30 (17%) of the patients with VVC and in seven of ten (70%) patients with RVVC (p=0.003). Isolates from nineteen patients were proteinase positive. Proteinase production and type of treatment were not related to recurrence of VVC (p>0.05). DNA typing revealed that in this population RVVC might be due to the same strain, substrain shuffling or different strains and species.

Original languageEnglish (US)
Pages (from-to)89-94
Number of pages6
JournalRevista Iberoamericana de Micologia
Volume19
Issue number2
StatePublished - 2002
Externally publishedYes

Fingerprint

Vulvovaginal Candidiasis
DNA Fingerprinting
Candida
Peptide Hydrolases
Recurrence
Fluconazole
Itraconazole
Candida albicans
Disk Diffusion Antimicrobial Tests
Karyotyping
Sexual Partners
Pulsed Field Gel Electrophoresis
Restriction Fragment Length Polymorphisms
Therapeutics
Population

Keywords

  • DNA typing
  • MIC
  • Proteinase
  • Vulvovaginal candidiasis

ASJC Scopus subject areas

  • Microbiology

Cite this

Proteinase detection, DNA typing and antimycotic susceptibility of Candida isolates from Colombian women with vulvovaginal candidiasis. / Cardona-Castro, Nora; Revankar, Sanjay G.; Ortiz, Patricia; Cuervo, Claudia; Kirkpatrick, William R.; McAtee, Robert K.; Patterson, Thomas F.

In: Revista Iberoamericana de Micologia, Vol. 19, No. 2, 2002, p. 89-94.

Research output: Contribution to journalArticle

Cardona-Castro, Nora ; Revankar, Sanjay G. ; Ortiz, Patricia ; Cuervo, Claudia ; Kirkpatrick, William R. ; McAtee, Robert K. ; Patterson, Thomas F. / Proteinase detection, DNA typing and antimycotic susceptibility of Candida isolates from Colombian women with vulvovaginal candidiasis. In: Revista Iberoamericana de Micologia. 2002 ; Vol. 19, No. 2. pp. 89-94.
@article{c1cb7eb283844ea496c916a0dcf5b9db,
title = "Proteinase detection, DNA typing and antimycotic susceptibility of Candida isolates from Colombian women with vulvovaginal candidiasis",
abstract = "Forty non-pregnant Colombian women (ages 18 - 45) with vulvovaginal candidiasis diagnosis (VVC) were enrolled in a blinded study to compare the efficacy of Itraconazole (ITRA) 400 mg vs. Fluconazole (FLU) 150 mg. Sexual partners received similar therapy. Proteinase detection by the Staib method and minimal inhibitory concentration (MIC) for FLU and ITRA by Etest method were performed in all Candida isolates. Patients were followed one year to determine clinical evolution and recurrence of VVC (RVVC). The strain identity of the RVVC isolates was determined by contour-clamped homogeneous electric field (CHEF) gel electrophoresis karyotyping and restriction fragment length polymorphism (RFLP). Thirty patients (75{\%}) had one or two episodes of VVC/year, 83{\%} of these were due to Candida albicans, while ten patients (25{\%}) developed RVVC (three or more episodes/year); seven of them were treated with FLU. Non-C. albicans Candida species were detected in five of 30 (17{\%}) of the patients with VVC and in seven of ten (70{\%}) patients with RVVC (p=0.003). Isolates from nineteen patients were proteinase positive. Proteinase production and type of treatment were not related to recurrence of VVC (p>0.05). DNA typing revealed that in this population RVVC might be due to the same strain, substrain shuffling or different strains and species.",
keywords = "DNA typing, MIC, Proteinase, Vulvovaginal candidiasis",
author = "Nora Cardona-Castro and Revankar, {Sanjay G.} and Patricia Ortiz and Claudia Cuervo and Kirkpatrick, {William R.} and McAtee, {Robert K.} and Patterson, {Thomas F}",
year = "2002",
language = "English (US)",
volume = "19",
pages = "89--94",
journal = "Revista Iberoamericana de Micologia",
issn = "1130-1406",
publisher = "Asociacion Espanola de Micologia",
number = "2",

}

TY - JOUR

T1 - Proteinase detection, DNA typing and antimycotic susceptibility of Candida isolates from Colombian women with vulvovaginal candidiasis

AU - Cardona-Castro, Nora

AU - Revankar, Sanjay G.

AU - Ortiz, Patricia

AU - Cuervo, Claudia

AU - Kirkpatrick, William R.

AU - McAtee, Robert K.

AU - Patterson, Thomas F

PY - 2002

Y1 - 2002

N2 - Forty non-pregnant Colombian women (ages 18 - 45) with vulvovaginal candidiasis diagnosis (VVC) were enrolled in a blinded study to compare the efficacy of Itraconazole (ITRA) 400 mg vs. Fluconazole (FLU) 150 mg. Sexual partners received similar therapy. Proteinase detection by the Staib method and minimal inhibitory concentration (MIC) for FLU and ITRA by Etest method were performed in all Candida isolates. Patients were followed one year to determine clinical evolution and recurrence of VVC (RVVC). The strain identity of the RVVC isolates was determined by contour-clamped homogeneous electric field (CHEF) gel electrophoresis karyotyping and restriction fragment length polymorphism (RFLP). Thirty patients (75%) had one or two episodes of VVC/year, 83% of these were due to Candida albicans, while ten patients (25%) developed RVVC (three or more episodes/year); seven of them were treated with FLU. Non-C. albicans Candida species were detected in five of 30 (17%) of the patients with VVC and in seven of ten (70%) patients with RVVC (p=0.003). Isolates from nineteen patients were proteinase positive. Proteinase production and type of treatment were not related to recurrence of VVC (p>0.05). DNA typing revealed that in this population RVVC might be due to the same strain, substrain shuffling or different strains and species.

AB - Forty non-pregnant Colombian women (ages 18 - 45) with vulvovaginal candidiasis diagnosis (VVC) were enrolled in a blinded study to compare the efficacy of Itraconazole (ITRA) 400 mg vs. Fluconazole (FLU) 150 mg. Sexual partners received similar therapy. Proteinase detection by the Staib method and minimal inhibitory concentration (MIC) for FLU and ITRA by Etest method were performed in all Candida isolates. Patients were followed one year to determine clinical evolution and recurrence of VVC (RVVC). The strain identity of the RVVC isolates was determined by contour-clamped homogeneous electric field (CHEF) gel electrophoresis karyotyping and restriction fragment length polymorphism (RFLP). Thirty patients (75%) had one or two episodes of VVC/year, 83% of these were due to Candida albicans, while ten patients (25%) developed RVVC (three or more episodes/year); seven of them were treated with FLU. Non-C. albicans Candida species were detected in five of 30 (17%) of the patients with VVC and in seven of ten (70%) patients with RVVC (p=0.003). Isolates from nineteen patients were proteinase positive. Proteinase production and type of treatment were not related to recurrence of VVC (p>0.05). DNA typing revealed that in this population RVVC might be due to the same strain, substrain shuffling or different strains and species.

KW - DNA typing

KW - MIC

KW - Proteinase

KW - Vulvovaginal candidiasis

UR - http://www.scopus.com/inward/record.url?scp=0036067084&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036067084&partnerID=8YFLogxK

M3 - Article

C2 - 12828510

AN - SCOPUS:0036067084

VL - 19

SP - 89

EP - 94

JO - Revista Iberoamericana de Micologia

JF - Revista Iberoamericana de Micologia

SN - 1130-1406

IS - 2

ER -