Protein tyrosine phosphatases PTP-1B, SHP-2, and PTEN facilitate Rb/E2F-associated apoptotic signaling

Liza D. Morales, Edgar A. Casillas Pavón, Jun Wan Shin, Alexander Garcia, Mario Capetillo, Dae Joon Kim, Jonathan H. Lieman

Research output: Contribution to journalArticle

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Abstract

To maintain tissue homeostasis, apoptosis is functionally linked to the cell cycle through the retinoblastoma (Rb)/E2F pathway. When the Rb tumor suppressor protein is functionally inactivated, E2F1 elicits an apoptotic response through both intrinsic (caspase-9 mediated) and extrinsic (caspase-8 mediated) apoptotic pathways in order to eliminate hyperproliferative cells. Rb/E2F-associated apoptosis has been demonstrated to be associated with the loss of constitutive transcriptional repression by Rb/E2F complexes and mediated by caspase-8. Protein tyrosine phosphatases (PTPs) PTP-1B and SHP-2 have been previously shown to be directly activated by loss of Rb/E2F repression during Rb/E2F-associated apoptosis. In this current study, we demonstrate that the PTEN tumor suppressor is also directly activated by loss of Rb/E2F repression. We also demonstrate that PTP-1B, SHP-2, and PTEN play a functional role in Rb/E2F-associated apoptosis. Knockdown of PTP1B, SHP2, or PTEN expression with small interfering RNA (siRNA) in apoptotic cells increases cell viability and rescues cells from the Rb/E2F-associated apoptotic response. Furthermore, rescue from apoptosis coincides with inhibition of caspase-8 and caspase-3 cleavage (activation). Our results indicate PTP-1B, SHP-2, and PTEN all play a functional role in Rb/E2F-associated apoptotic signal transduction and provide further evidence that PTP-1B, SHP-2, and PTEN can contribute to tumor suppression through an Rb/E2F-associated mechanism.

Original languageEnglish (US)
Article numbere97104
JournalPLoS One
Volume9
Issue number5
DOIs
StatePublished - May 8 2014

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Non-Receptor Type 1 Protein Tyrosine Phosphatase
protein-tyrosine-phosphatase
Protein Tyrosine Phosphatases
Retinoblastoma
caspase-8
apoptosis
Caspase 8
Apoptosis
Cells
Tumors
Tissue homeostasis
Tumor Suppressor Proteins
caspase-9
Signal transduction
neoplasms
Caspase 9
cells
caspase-3
small interfering RNA
Caspase 3

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Morales, L. D., Casillas Pavón, E. A., Shin, J. W., Garcia, A., Capetillo, M., Kim, D. J., & Lieman, J. H. (2014). Protein tyrosine phosphatases PTP-1B, SHP-2, and PTEN facilitate Rb/E2F-associated apoptotic signaling. PLoS One, 9(5), [e97104]. https://doi.org/10.1371/journal.pone.0097104

Protein tyrosine phosphatases PTP-1B, SHP-2, and PTEN facilitate Rb/E2F-associated apoptotic signaling. / Morales, Liza D.; Casillas Pavón, Edgar A.; Shin, Jun Wan; Garcia, Alexander; Capetillo, Mario; Kim, Dae Joon; Lieman, Jonathan H.

In: PLoS One, Vol. 9, No. 5, e97104, 08.05.2014.

Research output: Contribution to journalArticle

Morales, LD, Casillas Pavón, EA, Shin, JW, Garcia, A, Capetillo, M, Kim, DJ & Lieman, JH 2014, 'Protein tyrosine phosphatases PTP-1B, SHP-2, and PTEN facilitate Rb/E2F-associated apoptotic signaling', PLoS One, vol. 9, no. 5, e97104. https://doi.org/10.1371/journal.pone.0097104
Morales LD, Casillas Pavón EA, Shin JW, Garcia A, Capetillo M, Kim DJ et al. Protein tyrosine phosphatases PTP-1B, SHP-2, and PTEN facilitate Rb/E2F-associated apoptotic signaling. PLoS One. 2014 May 8;9(5). e97104. https://doi.org/10.1371/journal.pone.0097104
Morales, Liza D. ; Casillas Pavón, Edgar A. ; Shin, Jun Wan ; Garcia, Alexander ; Capetillo, Mario ; Kim, Dae Joon ; Lieman, Jonathan H. / Protein tyrosine phosphatases PTP-1B, SHP-2, and PTEN facilitate Rb/E2F-associated apoptotic signaling. In: PLoS One. 2014 ; Vol. 9, No. 5.
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