Protein synthesis inhibitor-mediated stability of adenomatous polyposis coli mRNA levels in HCT-116 colon cancer cells

Aruna S. Jaiswal, Satya Narayan

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

We examined the effect of a protein synthesis inhibitor, cycloheximide (CHX) on the adenomatous polyposis coli (APC) mRNA levels in HCT-116 colon cancer cell line. The HCT-116 cells were treated with different concentrations of CHX for 15 h. APC, p53 and β-actin mRNA levels were determined by Northern blotting. Results showed that APC and β-actin mRNA levels were significantly increased in a dose-dependent manner after CHX treatment. The p53 mRNA levels were moderately increased. The increase in APC mRNA levels after CHX treatment was due to increase in its stability instead of transcription. These results provide a model for CHX-induced APC mRNA stabilization and its implication in cell cycle arrest and cell survival studies.

Original languageEnglish (US)
Pages (from-to)1045-1048
Number of pages4
JournalInternational journal of oncology
Volume14
Issue number6
DOIs
StatePublished - Jun 1999
Externally publishedYes

Keywords

  • Adenomatous polyposis coli (APC) gene expression
  • Colon cancer
  • Cycloheximide
  • Transcription
  • mRNA stability

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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