A Millipore filtration assay method has been developed for studies of codon directed Met-tRNAfMet binding to 40S ribosomes. Under the assay conditions, the stimulation of Met-tRNAfMet binding to Millipore filters by added 40S ribosomes is strongly dependent on the presence of AUG codon, and partially purified peptide chain initiation factors. Crude 0.5 M KC1 ribosomal wash is inactive in catalyzing this binding reaction due to the presence of an inhibitor in this preparation.
|Original language||English (US)|
|Number of pages||9|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Jul 22 1975|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology