Protein synthesis in rabbit reticulocytes XII: Requirement of mRNA (AUG codon) for Met-tRNAfMet binding to 40S ribosomes

N. K. Gupta; B. Chatterjee; A. Majumdar

doi:10.1016/S0006-291X(75)80215-4

Protein synthesis in rabbit reticulocytes XII: Requirement of mRNA (AUG codon) for Met-tRNA_f^Met binding to 40S ribosomes

N. K. Gupta, B. Chatterjee, A. Majumdar

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

A Millipore filtration assay method has been developed for studies of codon directed Met-tRNA_f^Met binding to 40S ribosomes. Under the assay conditions, the stimulation of Met-tRNA_f^Met binding to Millipore filters by added 40S ribosomes is strongly dependent on the presence of AUG codon, and partially purified peptide chain initiation factors. Crude 0.5 M KC1 ribosomal wash is inactive in catalyzing this binding reaction due to the presence of an inhibitor in this preparation.

Original language	English (US)
Pages (from-to)	797-805
Number of pages	9
Journal	Biochemical and Biophysical Research Communications
Volume	65
Issue number	2
DOIs	https://doi.org/10.1016/S0006-291X(75)80215-4
State	Published - Jul 22 1975
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Biophysics
Biochemistry
Cell Biology

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title = "Protein synthesis in rabbit reticulocytes XII: Requirement of mRNA (AUG codon) for Met-tRNAfMet binding to 40S ribosomes",

abstract = "A Millipore filtration assay method has been developed for studies of codon directed Met-tRNAfMet binding to 40S ribosomes. Under the assay conditions, the stimulation of Met-tRNAfMet binding to Millipore filters by added 40S ribosomes is strongly dependent on the presence of AUG codon, and partially purified peptide chain initiation factors. Crude 0.5 M KC1 ribosomal wash is inactive in catalyzing this binding reaction due to the presence of an inhibitor in this preparation.",

author = "Gupta, {N. K.} and B. Chatterjee and A. Majumdar",

note = "Funding Information: This work was supported by NIH Research Grants (GM-18796, GM-22079) and an American Cancer Society Grant NP-I13. The authors thank Mr. Y. C. Chen for generous supplies of DNA-template and RNA polymerase.",

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T2 - Requirement of mRNA (AUG codon) for Met-tRNAfMet binding to 40S ribosomes

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AU - Chatterjee, B.

AU - Majumdar, A.

N1 - Funding Information: This work was supported by NIH Research Grants (GM-18796, GM-22079) and an American Cancer Society Grant NP-I13. The authors thank Mr. Y. C. Chen for generous supplies of DNA-template and RNA polymerase.

PY - 1975/7/22

Y1 - 1975/7/22

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AB - A Millipore filtration assay method has been developed for studies of codon directed Met-tRNAfMet binding to 40S ribosomes. Under the assay conditions, the stimulation of Met-tRNAfMet binding to Millipore filters by added 40S ribosomes is strongly dependent on the presence of AUG codon, and partially purified peptide chain initiation factors. Crude 0.5 M KC1 ribosomal wash is inactive in catalyzing this binding reaction due to the presence of an inhibitor in this preparation.

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Protein synthesis in rabbit reticulocytes XII: Requirement of mRNA (AUG codon) for Met-tRNA_f^Met binding to 40S ribosomes

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