Protein synthesis in rabbit reticulocytes XII: Requirement of mRNA (AUG codon) for Met-tRNAfMet binding to 40S ribosomes

N. K. Gupta, B. Chatterjee, A. Majumdar

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

A Millipore filtration assay method has been developed for studies of codon directed Met-tRNAfMet binding to 40S ribosomes. Under the assay conditions, the stimulation of Met-tRNAfMet binding to Millipore filters by added 40S ribosomes is strongly dependent on the presence of AUG codon, and partially purified peptide chain initiation factors. Crude 0.5 M KC1 ribosomal wash is inactive in catalyzing this binding reaction due to the presence of an inhibitor in this preparation.

Original languageEnglish (US)
Pages (from-to)797-805
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume65
Issue number2
DOIs
StatePublished - Jul 22 1975
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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