Protein synthesis in rabbit reticulocytes: Evidence for two forms of the Met-tRNAfMet binding factors

N. K. Gupta; B. Chatterjee; Y. C. Chen; A. Majumdar

doi:10.1016/S0006-291X(74)80474-2

Protein synthesis in rabbit reticulocytes: Evidence for two forms of the Met-tRNA_f^Met binding factors

N. K. Gupta
, B. Chatterjee
, Y. C. Chen
, A. Majumdar

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Two forms of Met-tRNA_f^Met binding factors (IF1A and IF1B) were seperated by DEAE-cellulose chromatography. These two forms can be distinguished by the stabilities of their respective Met-tRNA_f^Met:IF1:GTP complexes to Mg⁺⁺. The Met-tRNA_f^Met:IF1B:GTP complex dissociates extensively in the presence of Mg⁺⁺ where as the Met-tRNA_f^Met:IF1A:GTP complex is distinctly more stable under similar conditions. The molecular weight of Met-tRNA_f^Met:IF1A:GTP complex as determined by sucrose density gradient centrifugation is 160,000 and that of the Met-tRNA_f^Met:IF1B:GTP complex is 65,000.

Original language	English (US)
Pages (from-to)	699-706
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	58
Issue number	3
DOIs	https://doi.org/10.1016/S0006-291X(74)80474-2
State	Published - Jun 4 1974
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Biophysics
Biochemistry
Cell Biology

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10.1016/S0006-291X(74)80474-2

Cite this

@article{235af71e28744dcf98dca6406a66aefd,

title = "Protein synthesis in rabbit reticulocytes: Evidence for two forms of the Met-tRNAfMet binding factors",

abstract = "Two forms of Met-tRNAfMet binding factors (IF1A and IF1B) were seperated by DEAE-cellulose chromatography. These two forms can be distinguished by the stabilities of their respective Met-tRNAfMet:IF1:GTP complexes to Mg++. The Met-tRNAfMet:IF1B:GTP complex dissociates extensively in the presence of Mg++ where as the Met-tRNAfMet:IF1A:GTP complex is distinctly more stable under similar conditions. The molecular weight of Met-tRNAfMet:IF1A:GTP complex as determined by sucrose density gradient centrifugation is 160,000 and that of the Met-tRNAfMet:IF1B:GTP complex is 65,000.",

author = "Gupta, \{N. K.\} and B. Chatterjee and Chen, \{Y. C.\} and A. Majumdar",

note = "Funding Information: Cancer Society Grant NP-II3 and a U.S. Public Health Service Career Development Funding Information: This work was supported by a NIH Research Grant (GM-18796), an American",

year = "1974",

month = jun,

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doi = "10.1016/S0006-291X(74)80474-2",

language = "English (US)",

volume = "58",

pages = "699--706",

journal = "Biochemical and Biophysical Research Communications",

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publisher = "Elsevier B.V.",

number = "3",

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TY - JOUR

T1 - Protein synthesis in rabbit reticulocytes

T2 - Evidence for two forms of the Met-tRNAfMet binding factors

AU - Gupta, N. K.

AU - Chatterjee, B.

AU - Chen, Y. C.

AU - Majumdar, A.

N1 - Funding Information: Cancer Society Grant NP-II3 and a U.S. Public Health Service Career Development Funding Information: This work was supported by a NIH Research Grant (GM-18796), an American

PY - 1974/6/4

Y1 - 1974/6/4

N2 - Two forms of Met-tRNAfMet binding factors (IF1A and IF1B) were seperated by DEAE-cellulose chromatography. These two forms can be distinguished by the stabilities of their respective Met-tRNAfMet:IF1:GTP complexes to Mg++. The Met-tRNAfMet:IF1B:GTP complex dissociates extensively in the presence of Mg++ where as the Met-tRNAfMet:IF1A:GTP complex is distinctly more stable under similar conditions. The molecular weight of Met-tRNAfMet:IF1A:GTP complex as determined by sucrose density gradient centrifugation is 160,000 and that of the Met-tRNAfMet:IF1B:GTP complex is 65,000.

AB - Two forms of Met-tRNAfMet binding factors (IF1A and IF1B) were seperated by DEAE-cellulose chromatography. These two forms can be distinguished by the stabilities of their respective Met-tRNAfMet:IF1:GTP complexes to Mg++. The Met-tRNAfMet:IF1B:GTP complex dissociates extensively in the presence of Mg++ where as the Met-tRNAfMet:IF1A:GTP complex is distinctly more stable under similar conditions. The molecular weight of Met-tRNAfMet:IF1A:GTP complex as determined by sucrose density gradient centrifugation is 160,000 and that of the Met-tRNAfMet:IF1B:GTP complex is 65,000.

UR - https://www.scopus.com/pages/publications/0016254445

UR - https://www.scopus.com/pages/publications/0016254445#tab=citedBy

U2 - 10.1016/S0006-291X(74)80474-2

DO - 10.1016/S0006-291X(74)80474-2

M3 - Article

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AN - SCOPUS:0016254445

SN - 0006-291X

VL - 58

SP - 699

EP - 706

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Protein synthesis in rabbit reticulocytes: Evidence for two forms of the Met-tRNA_f^Met binding factors

Abstract

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