Two forms of Met-tRNAfMet binding factors (IF1A and IF1B) were seperated by DEAE-cellulose chromatography. These two forms can be distinguished by the stabilities of their respective Met-tRNAfMet:IF1:GTP complexes to Mg++. The Met-tRNAfMet:IF1B:GTP complex dissociates extensively in the presence of Mg++ where as the Met-tRNAfMet:IF1A:GTP complex is distinctly more stable under similar conditions. The molecular weight of Met-tRNAfMet:IF1A:GTP complex as determined by sucrose density gradient centrifugation is 160,000 and that of the Met-tRNAfMet:IF1B:GTP complex is 65,000.
|Original language||English (US)|
|Number of pages||8|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Jun 4 1974|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology