Protein synthesis in rabbit reticulocytes: Evidence for two forms of the Met-tRNAfMet binding factors

N. K. Gupta, B. Chatterjee, Y. C. Chen, A. Majumdar

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Two forms of Met-tRNAfMet binding factors (IF1A and IF1B) were seperated by DEAE-cellulose chromatography. These two forms can be distinguished by the stabilities of their respective Met-tRNAfMet:IF1:GTP complexes to Mg++. The Met-tRNAfMet:IF1B:GTP complex dissociates extensively in the presence of Mg++ where as the Met-tRNAfMet:IF1A:GTP complex is distinctly more stable under similar conditions. The molecular weight of Met-tRNAfMet:IF1A:GTP complex as determined by sucrose density gradient centrifugation is 160,000 and that of the Met-tRNAfMet:IF1B:GTP complex is 65,000.

Original languageEnglish (US)
Pages (from-to)699-706
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume58
Issue number3
DOIs
StatePublished - Jun 4 1974
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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