Protein synthesis during early adult and second pupal development in the silkmoth, Antheraea polyphemus

Robert G. Brzyski, Mary F. Ruh

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Experiments were performed to measure protein synthesis in wing epidermal cells during early pharate adult and second pupal development. The period for juvenile hormone (JH) determination of second pupal wings occurred between 18 and 24 hr after injection of 20-hydroxyecdysone (20-HE) plus JH. Between 12 and 24 hr, [35S]-methionine incorporation remained relatively constant in pupae that had received exogenous hormones (either 20-HE alone or 20-HE and JH) and there was no significant difference between these groups. For 12-48 hr after hormone injection, no variation in banding patterns specific to a particular hormone treatment were evident by one-dimensional electrophoresis, despite dramatic changes in protein content at this time. At 3-6 days, differentiation specific protein bands were detected. Two dimensional electrophoresis was then used to examine more closely specific proteins synthesized during the 18-24 period, when developmental reprogramming occurred. At 24 hr, the synthesis of two clusters of proteins and a single protein were detected in samples from 20-HE treated animals but were not detected in samples from animals injected with 20-HE plus JH. No JH-specific proteins were detected. The adult specific proteins may be the product of events concerning the mechanisms of adult commitment.

Original languageEnglish (US)
Pages (from-to)687-698
Number of pages12
JournalInsect Biochemistry
Volume12
Issue number6
DOIs
StatePublished - 1982
Externally publishedYes

Keywords

  • 20-OH-ecdysone
  • juvenile hormone
  • protein synthesis

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