@inproceedings{4997e0ed47514bd28be9f77fc1117c2a,
title = "Protein-protein binding before and after photo-modification of albumin",
abstract = "Bioeffects of directed-optical-energy encompass a wide range of applications. One aspect of photochemical interactions involves irradiating a photosensitizer with visible light in order to induce protein unfolding and consequent changes in function. In the past, irradiation of several dye-protein combinations has revealed effects on protein structure. Beta lactoglobulin, human serum albumin (HSA) and tubulin have all been photo-modified with meso-tetrakis(4-sulfonatophenyl)porphyrin (TSPP) bound, but only in the case of tubulin has binding caused a verified loss of biological function (loss of ability to form microtubules) as a result of this light-induced structural change. The current work questions if the photo-induced structural changes that occur to HSA, are sufficient to disable its biological function of binding to osteonectin. The albumin-binding protein, osteonectin, is about half the molecular weight of HSA, so the two proteins and their bound product can be separated and quantified by size exclusion high performance liquid chromatography. TSPP was first bound to HSA and irradiated, photo-modifying the structure of HSA. Then native HSA or photo-modified HSA (both with TSPP bound) were compared, to assess loss in HSA's innate binding ability as a result of light-induced structure modification.",
keywords = "HPLC, HSA, SPARC, albumin, osteonectin, photosensitizer, protein-protein binding, size exclusion chromatography",
author = "Rozinek, {Sarah C.} and Glickman, {Randolph D.} and Thomas, {Robert J.} and Lorenzo Brancaleon",
note = "Funding Information: S.C.R is supported by the Consortium Research Fellows Program (FA8650-13-2-6333). The research is funded by a combination of an AFOSR grant (FA8650-07-D-6800) to R.J.T. and an NIH grant (G12RR013646-1) to L.B. Additionally, S.C.R. wishes to thank the co-authors of this work, as well as Morgan Schmidt (Engility) for their research. Pending Distribution A: Approved for public release; distribution unlimited. STINFO PA Case No: RH-16-115075 Publisher Copyright: {\textcopyright} 2016 SPIE. Copyright: Copyright 2016 Elsevier B.V., All rights reserved.; Optical Interactions with Tissue and Cells XXVII ; Conference date: 14-02-2016 Through 17-02-2016",
year = "2016",
doi = "10.1117/12.2213632",
language = "English (US)",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
editor = "Jansen, {E. Duco}",
booktitle = "Optical Interactions with Tissue and Cells XXVII",
}