Protein interactions with polylactide-polyglycolide particles and scaffolds

John P. Schmitz, Gustavo Zardeneta, C. Mauli Agrawal, Tim Lee, Kyriacos Athanasiou, Stephen B. Milam

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Biodegradable implants of polylactide and polyglycolide are increasingly used in tissue engineering as well as alternatives to the use of metal plates and screws in the rigid fixation of fractures. Contact with proteins is the first biological event that may influence dynamic cellular interactions with these materials. In this study, the biophysical effects of protein interactions with poly (D,L-lactide)-co-glycolide (PLG) particles and scaffolds were examined using a model protein, xanthine oxidase (XO). Adsorption of XO to PLG particles or scaffolds caused a loss of activity of the XO, while preadsorbing bovine serum albumin to particles appeared to prevent loss of protein activity. Surface fluorescence of XO bound to PLG particles and scaffolds showed that XO was partially denatured. Furthermore, XO bound to PLG scaffolds was found to exist in a more native configuration than XO bound to PLG particles. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of adsorption of serum proteins to PLG demonstrated three major bands, one of which may be albumin based on isoelectric point and molecular weight. Therefore, it can be concluded from this study that certain enzymes may become irreversibly inactivated when bound to PLG particles or scaffolds.

Original languageEnglish (US)
Pages (from-to)257-266
Number of pages10
JournalTissue Engineering
Volume3
Issue number3
DOIs
StatePublished - 1997

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Polyglycolic Acid
Xanthine Oxidase
Scaffolds (biology)
Proteins
Scaffolds
Adsorption
Absorbable Implants
Fracture Fixation
Plate metal
Isoelectric Point
Sodium dodecyl sulfate
Tissue Engineering
Bovine Serum Albumin
poly(lactide)
Oxidoreductases
Polyacrylates
Electrophoresis
Tissue engineering
Sodium Dodecyl Sulfate
Particles (particulate matter)

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology

Cite this

Schmitz, J. P., Zardeneta, G., Agrawal, C. M., Lee, T., Athanasiou, K., & Milam, S. B. (1997). Protein interactions with polylactide-polyglycolide particles and scaffolds. Tissue Engineering, 3(3), 257-266. https://doi.org/10.1089/ten.1997.3.257

Protein interactions with polylactide-polyglycolide particles and scaffolds. / Schmitz, John P.; Zardeneta, Gustavo; Agrawal, C. Mauli; Lee, Tim; Athanasiou, Kyriacos; Milam, Stephen B.

In: Tissue Engineering, Vol. 3, No. 3, 1997, p. 257-266.

Research output: Contribution to journalArticle

Schmitz, JP, Zardeneta, G, Agrawal, CM, Lee, T, Athanasiou, K & Milam, SB 1997, 'Protein interactions with polylactide-polyglycolide particles and scaffolds', Tissue Engineering, vol. 3, no. 3, pp. 257-266. https://doi.org/10.1089/ten.1997.3.257
Schmitz JP, Zardeneta G, Agrawal CM, Lee T, Athanasiou K, Milam SB. Protein interactions with polylactide-polyglycolide particles and scaffolds. Tissue Engineering. 1997;3(3):257-266. https://doi.org/10.1089/ten.1997.3.257
Schmitz, John P. ; Zardeneta, Gustavo ; Agrawal, C. Mauli ; Lee, Tim ; Athanasiou, Kyriacos ; Milam, Stephen B. / Protein interactions with polylactide-polyglycolide particles and scaffolds. In: Tissue Engineering. 1997 ; Vol. 3, No. 3. pp. 257-266.
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