Protein and amino acid supplementation does not alter proteolytic gene expression following immobilization

Jennifer A. Bunn, Thomas W. Buford, Monica C. Serra, Richard B. Kreider, Darryn S. Willoughby

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Objective. To determine if supplementation of protein and amino acids (PAA) decreases skeletal muscle expression of atrophy-related genes, muscle mass, and strength during immobilization in humans. Methods. Twenty males wore a lower-limb immobilization boot for 28 days and consumed either a PAA supplement (28 g protein) or carbohydrate placebo (28 g maltodextrose), while consuming their normal daily diet. Testing sessions included dietary analysis, lower-leg girth and body composition measurements, strength testing, and gastrocnemius muscle biopsies. Muscle was analyzed for mRNA expression of markers in the ubiquitin and calpain systems, myostatin, TNF- and NF-B. Results. All genes of interest increased over time (P <.05), but there was no difference between groups. Lower-leg girth decreased over time (P = 0.02); however, there were no significant changes in body composition or strength. Conclusion. Short-term lower-limb disuse, despite the absence of significant muscle atrophy, is associated with increases in skeletal muscle gene expression of several proteolysis-related genes. These changes do not appear to be altered by oral PAA supplementation.

Original languageEnglish (US)
Article number539690
JournalJournal of Nutrition and Metabolism
Volume2011
DOIs
StatePublished - 2011
Externally publishedYes

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Food Science
  • Nutrition and Dietetics

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