TY - JOUR
T1 - Protective effects of resveratrol on TNF-α-induced endothelial cytotoxicity in baboon femoral arterial endothelial cells
AU - Xiao, Juan
AU - Song, Jun
AU - Hodara, Vida
AU - Ford, Allen
AU - Wang, Xing Li
AU - Shi, Qiang
AU - Chen, Li
AU - Vandeberg, John L.
N1 - Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2013
Y1 - 2013
N2 - Endothelial injury induced by inflammatory factors plays a critical role in the pathogenesis of cardiovascular disease. Endothelial cell (EC) apoptosis, proliferation, migration, and cellular adhesion molecule (CAM) expression contribute to the development of atherosclerosis. We investigated the effects of resveratrol (0.1-100 M) on the proliferation, migration, and CAM expression of primary cultures of baboon arterial endothelial cells (BAECs). In addition, we tested its effects under normal conditions as well as under inflammatory conditions induced by tumour necrosis factor- α (TNF- α) administered either by cotreatment, pretreatment, or posttreatment. Immunocytochemistry, MTT, wound-healing, and flow cytometry assays were performed. The resveratrol treatment significantly enhanced BAEC proliferation and attenuated TNF- α -induced impairment of proliferation at the optimal doses of 1-50 μM. Resveratrol at a high dose (100 M) and TNF- α impaired BAEC migration, while low doses of resveratrol (1-50 M) attenuated TNF- α -induced impairment of BAEC migration. Moreover, resveratrol inhibited TNF- α -induced ICAM-1 and VCAM-1 expression. Taken together, our results suggest that the resveratrol protects BAECs after inflammatory stimulation as well as ameliorates inflammatory effects at low concentrations. Consequently, resveratrol should be considered as a candidate drug for the prevention and treatment of inflammatory vascular diseases.
AB - Endothelial injury induced by inflammatory factors plays a critical role in the pathogenesis of cardiovascular disease. Endothelial cell (EC) apoptosis, proliferation, migration, and cellular adhesion molecule (CAM) expression contribute to the development of atherosclerosis. We investigated the effects of resveratrol (0.1-100 M) on the proliferation, migration, and CAM expression of primary cultures of baboon arterial endothelial cells (BAECs). In addition, we tested its effects under normal conditions as well as under inflammatory conditions induced by tumour necrosis factor- α (TNF- α) administered either by cotreatment, pretreatment, or posttreatment. Immunocytochemistry, MTT, wound-healing, and flow cytometry assays were performed. The resveratrol treatment significantly enhanced BAEC proliferation and attenuated TNF- α -induced impairment of proliferation at the optimal doses of 1-50 μM. Resveratrol at a high dose (100 M) and TNF- α impaired BAEC migration, while low doses of resveratrol (1-50 M) attenuated TNF- α -induced impairment of BAEC migration. Moreover, resveratrol inhibited TNF- α -induced ICAM-1 and VCAM-1 expression. Taken together, our results suggest that the resveratrol protects BAECs after inflammatory stimulation as well as ameliorates inflammatory effects at low concentrations. Consequently, resveratrol should be considered as a candidate drug for the prevention and treatment of inflammatory vascular diseases.
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U2 - 10.1155/2013/185172
DO - 10.1155/2013/185172
M3 - Article
C2 - 23671856
AN - SCOPUS:84879347868
VL - 2013
JO - Journal of Diabetes Research
JF - Journal of Diabetes Research
SN - 2314-6745
M1 - 185172
ER -