Abstract
The aim of the study was to examine the potential protective effect of melatonin against whole body ionizing radiation (800 cGy). Changes in 8-hydroxy-2'-deoxyguanosine (8-OH-dG) levels, an index of DNA damage, and alterations in membrane fluidity (the inverse of membrane rigidity) and lipid peroxidation in microsomal membranes, as indices of damage to lipid and protein molecules in membranes, were estimated. Measurements were made in rat liver, 12 h after their exposure to radiation. To test the potential protective effects of melatonin, the indole was injected (i.p. 50 mg/kg b.w.) at 120, 90, 60 and 30 min prior to radiation exposure. Both 8-OH-dG levels and microsomal membrane rigidity increased significantly 12 h after radiation exposure. Melatonin completely counteracted the effects of ionizing radiation. Changes in 8-OH-dG levels and membrane fluidity are early sensitive parameters of DNA and microsomal membrane damage, respectively, induced by ionizing radiation and our findings document the protective effects of melatonin against ionizing radiation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 137-144 |
| Number of pages | 8 |
| Journal | Molecular and Cellular Biochemistry |
| Volume | 211 |
| Issue number | 1-2 |
| State | Published - 2000 |
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Keywords
- 8-hydroxy-2'-deoxyguanosine
- Free radicals
- Ionizing radiation
- Lipid peroxidation
- Melatonin
- Membrane fluidity
ASJC Scopus subject areas
- Clinical Biochemistry
- Molecular Biology
- Genetics
- Cell Biology
Cite this
Protective effects of melatonin against oxidation of guanine bases in DNA and decreased microsomal membrane fluidity in rat liver induced by whole body ionizing radiation. / Karbownik, M.; Reiter, Russel J; Qi, W.; Garcia, J. J.; Tan, D. X.; Manchester, L. C.; Vijayalaxmi.
In: Molecular and Cellular Biochemistry, Vol. 211, No. 1-2, 2000, p. 137-144.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Protective effects of melatonin against oxidation of guanine bases in DNA and decreased microsomal membrane fluidity in rat liver induced by whole body ionizing radiation
AU - Karbownik, M.
AU - Reiter, Russel J
AU - Qi, W.
AU - Garcia, J. J.
AU - Tan, D. X.
AU - Manchester, L. C.
AU - Vijayalaxmi,
PY - 2000
Y1 - 2000
N2 - The aim of the study was to examine the potential protective effect of melatonin against whole body ionizing radiation (800 cGy). Changes in 8-hydroxy-2'-deoxyguanosine (8-OH-dG) levels, an index of DNA damage, and alterations in membrane fluidity (the inverse of membrane rigidity) and lipid peroxidation in microsomal membranes, as indices of damage to lipid and protein molecules in membranes, were estimated. Measurements were made in rat liver, 12 h after their exposure to radiation. To test the potential protective effects of melatonin, the indole was injected (i.p. 50 mg/kg b.w.) at 120, 90, 60 and 30 min prior to radiation exposure. Both 8-OH-dG levels and microsomal membrane rigidity increased significantly 12 h after radiation exposure. Melatonin completely counteracted the effects of ionizing radiation. Changes in 8-OH-dG levels and membrane fluidity are early sensitive parameters of DNA and microsomal membrane damage, respectively, induced by ionizing radiation and our findings document the protective effects of melatonin against ionizing radiation.
AB - The aim of the study was to examine the potential protective effect of melatonin against whole body ionizing radiation (800 cGy). Changes in 8-hydroxy-2'-deoxyguanosine (8-OH-dG) levels, an index of DNA damage, and alterations in membrane fluidity (the inverse of membrane rigidity) and lipid peroxidation in microsomal membranes, as indices of damage to lipid and protein molecules in membranes, were estimated. Measurements were made in rat liver, 12 h after their exposure to radiation. To test the potential protective effects of melatonin, the indole was injected (i.p. 50 mg/kg b.w.) at 120, 90, 60 and 30 min prior to radiation exposure. Both 8-OH-dG levels and microsomal membrane rigidity increased significantly 12 h after radiation exposure. Melatonin completely counteracted the effects of ionizing radiation. Changes in 8-OH-dG levels and membrane fluidity are early sensitive parameters of DNA and microsomal membrane damage, respectively, induced by ionizing radiation and our findings document the protective effects of melatonin against ionizing radiation.
KW - 8-hydroxy-2'-deoxyguanosine
KW - Free radicals
KW - Ionizing radiation
KW - Lipid peroxidation
KW - Melatonin
KW - Membrane fluidity
UR - http://www.scopus.com/inward/record.url?scp=0033798101&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033798101&partnerID=8YFLogxK
M3 - Article
VL - 211
SP - 137
EP - 144
JO - Molecular and Cellular Biochemistry
JF - Molecular and Cellular Biochemistry
SN - 0300-8177
IS - 1-2
ER -