Protective effects of melatonin against carbon tetrachloride hepatotoxicity in rats

L. B. Zavodnik, Ilya B. Zavodnik, E. A. Lapshina, E. B. Belonovskaya, D. I. Martinchik, R. I. Kravchuk, M. Bryszewska, Russel J Reiter

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Melatonin is an indolamine, mainly secreted by the pineal gland into the blood of mammalian species. The potential for protective effects of melatonin on carbon tetrachloride (CCl4)-induced acute liver injury in rats was investigated in this work. CCl4 exerts its toxic effects by generation of free radicals; it was intragastrically administered to male Wistar rats (4 g kg-1 body weight) at 20 h before the animals were decapitated. Melatonin (15 mg kg-1 body weight) was administered intraperitoneally three times: 30 min before and at 2 and 4 h after CCl 4 injection. Rats injected with CCl4 alone showed significant lipid and hydropic dystrophy of the liver, massive necrosis of hepatocytes, marked increases in free and conjugated bilirubin levels, elevation of hepatic enzymes (alanine aminotransferase and aspartate aminotransferase) in plasma, as well as NO accumulation in liver and in blood. Melatonin administered at a pharmacological dose diminished the toxic effects of CCl 4. Thus it decreased both the structural and functional injury of hepatocytes and clearly exerted hepatoprotective effects. Melatonin administration also reduced CCl4-induced NO generation. These findings suggest that the effect of melatonin on CCl4-induced acute liver injury depends on the antioxidant action of melatonin.

Original languageEnglish (US)
Pages (from-to)353-359
Number of pages7
JournalCell Biochemistry and Function
Volume23
Issue number5
DOIs
StatePublished - Sep 2005

Fingerprint

Carbon Tetrachloride
Melatonin
Rats
Liver
Poisons
Hepatocytes
Wounds and Injuries
Blood
Body Weight
Cohort Effect
Pineal Gland
Aspartate Aminotransferases
Alanine Transaminase
Bilirubin
Free Radicals
Wistar Rats
Edema
Animals
Necrosis
Antioxidants

Keywords

  • Carbon tetrachloride
  • Lipid and hydropic dystrophy
  • Liver
  • Melatonin
  • Nitric oxide

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology

Cite this

Zavodnik, L. B., Zavodnik, I. B., Lapshina, E. A., Belonovskaya, E. B., Martinchik, D. I., Kravchuk, R. I., ... Reiter, R. J. (2005). Protective effects of melatonin against carbon tetrachloride hepatotoxicity in rats. Cell Biochemistry and Function, 23(5), 353-359. https://doi.org/10.1002/cbf.1160

Protective effects of melatonin against carbon tetrachloride hepatotoxicity in rats. / Zavodnik, L. B.; Zavodnik, Ilya B.; Lapshina, E. A.; Belonovskaya, E. B.; Martinchik, D. I.; Kravchuk, R. I.; Bryszewska, M.; Reiter, Russel J.

In: Cell Biochemistry and Function, Vol. 23, No. 5, 09.2005, p. 353-359.

Research output: Contribution to journalArticle

Zavodnik, LB, Zavodnik, IB, Lapshina, EA, Belonovskaya, EB, Martinchik, DI, Kravchuk, RI, Bryszewska, M & Reiter, RJ 2005, 'Protective effects of melatonin against carbon tetrachloride hepatotoxicity in rats', Cell Biochemistry and Function, vol. 23, no. 5, pp. 353-359. https://doi.org/10.1002/cbf.1160
Zavodnik LB, Zavodnik IB, Lapshina EA, Belonovskaya EB, Martinchik DI, Kravchuk RI et al. Protective effects of melatonin against carbon tetrachloride hepatotoxicity in rats. Cell Biochemistry and Function. 2005 Sep;23(5):353-359. https://doi.org/10.1002/cbf.1160
Zavodnik, L. B. ; Zavodnik, Ilya B. ; Lapshina, E. A. ; Belonovskaya, E. B. ; Martinchik, D. I. ; Kravchuk, R. I. ; Bryszewska, M. ; Reiter, Russel J. / Protective effects of melatonin against carbon tetrachloride hepatotoxicity in rats. In: Cell Biochemistry and Function. 2005 ; Vol. 23, No. 5. pp. 353-359.
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