Protective effect of β-carbolines and other antioxidants on lipid peroxidation due to hydrogen peroxide in rat brain homogenates

J. J. García, E. Martínez-Ballarín, M. Robinson, J. L. Allué, R. J. Reiter, C. Osuna, D. Acuña-Castroviejo

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Tryptoline and pinoline are two β-carbolines isolated from the nervous system of mammals. We investigated the ability of these compounds to prevent lipid peroxidation induced by hydrogen peroxide in rat brain homogenates. We also compared their effects with other known antioxidants including melatonin, trolox and ascorbic acid. Lipid peroxidation was assessed by measuring malonaldehyde (MDA) and 4-hydroxy-alkenals (4-HDA) concentrations in the brain homogenates. Incubation with hydrogen peroxide (5 mM) increased MDA+4-HDA levels, which were totally prevented by tryptoline, pinoline, melatonin and trolox in a concentration-dependent manner. By contrast, higher MDA-4-HDA concentrations compared with control experiments were found after incubation with ascorbic acid, thus reflecting an increase of lipid peroxidation induced by this compound. Although in vivo studies are needed, the data suggest that these β-carbolines may be potential neuroprotective agents because of their antioxidant activities. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish (US)
Pages (from-to)1-4
Number of pages4
JournalNeuroscience Letters
Volume294
Issue number1
DOIs
StatePublished - Nov 10 2000

Keywords

  • Brain homogenates
  • Hydrogen peroxide
  • Lipid peroxidation
  • Melatonin
  • Pinoline
  • Tryptoline

ASJC Scopus subject areas

  • Neuroscience(all)

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