Protection from rapamycin-induced apoptosis by insulin-like growth factor-I is partially dependent on protein kinase C signaling

Kuntebommanahalli N. Thimmaiah, John B. Easton, Peter J Houghton

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Rapamycin-induced apoptosis in sarcoma cells is inhibited by insulin-like growth factor-I (IGF-I) through a signaling pathway independent of Ras-extracellular signal-regulated kinase 1/2 and Akt. IGF-I induces Bad phosphorylation (Ser112, Ser136, and Ser155) in a pathway involving phosphoinositide 3′ kinase (PI3K) and protein kinase C (PKC; μ, ε, or θ) resulting in sequestering Bad from mitochondria and subsequently interacting with 14-3-3γ in the cytosol. Gene knockdown of Bad, Bid, Akt1, Akt2, PKC-μ, PKC-ε, or PKC-θ was achieved by transient transfection using small interfering RNAs. Results indicate that IGF-I signaling to Bad requires activation of PI3K and PKC (μ, θ, ε) but not mTOR, Ras-extracellular signal-regulated kinase 1/2, protein kinase A, or p90RSK. Wortmannin blocked the phosphorylation of PKC-μ (Ser744/Ser748), suggesting that PI3K is required for the activation of PKCs. PKCs phosphorylate Bad under in vitro conditions, and the association of phosphorylated Bad with PKC-μ or PKC-ε, as shown by immunoprecipitation, indicated direct involvement of PKCs in Bad phosphorylation. To confirm these results, cells overexpressing pEGFP-N1, wt-Bad, or Bad with a single site mutated (Ser112Ala; Ser136Ala; Ser155Ala), two sites mutated (Ser 112/136Ala; Ser112/155Ala; Ser136/155Ala), or the triple mutant were tested. IGF-I protected completely against rapamycin-induced apoptosis in cells overexpressing wt-Bad and mutants having either one or two sites of phosphorylation mutated. Knockdown of Bid using small interfering RNA showed that Bid is not required for rapamycin-induced cell death. Collectively, these data suggest that IGF-I-induced phosphorylation of Bad at multiple sites via a pathway involving PI3K and PKCs is important for protecting sarcoma cells from rapamycin-induced apoptosis.

Original languageEnglish (US)
Pages (from-to)2000-2009
Number of pages10
JournalCancer Research
Volume70
Issue number5
DOIs
StatePublished - Mar 1 2010
Externally publishedYes

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Sirolimus
Insulin-Like Growth Factor I
1-Phosphatidylinositol 4-Kinase
Protein Kinase C
Phosphorylation
Apoptosis
Mitogen-Activated Protein Kinase 3
Mitogen-Activated Protein Kinase 1
Sarcoma
Small Interfering RNA
Gene Knockdown Techniques
Cyclic AMP-Dependent Protein Kinases
Immunoprecipitation
Cytosol
Transfection
Mitochondria
Cell Death

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Protection from rapamycin-induced apoptosis by insulin-like growth factor-I is partially dependent on protein kinase C signaling. / Thimmaiah, Kuntebommanahalli N.; Easton, John B.; Houghton, Peter J.

In: Cancer Research, Vol. 70, No. 5, 01.03.2010, p. 2000-2009.

Research output: Contribution to journalArticle

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