Prostatic Nerve Growth Factor Inducible A Gene Binds a Novel Element in the Retinoblastoma Gene Promoter

Mark L. Day, Shixin Wu, Joseph W. Easier

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

We are investigating the role of the early response transcription factor, nerve growth factor inducible A gene (NGFI-A), as a modulator of retinoblastoma (RB) gene transcription in prostate cells. Examination of the RB promoter reveals a novel element GCGGGGGAG located at nucleotides 152-144 upstream of the methionine initiation codon. This sequence shares strong homology with the consensus NGFI-A binding element GCGGGGGCG varying by a single nucleotide. In DNA binding assays, an NGFI-A fusion protein and the native protein product of the NGFI-A gene purified from prostate cancer cells bound specifically to an oligonucleotide containing the RB promoter element. Gene expression studies in rat ventral prostate demonstrated a 1.9-fold increase in RB mRNA following castration that parallels a 2.7-fold induction of NGFI-A mRNA. In summary, the in vitro DNA binding data and the transient coregulation of rat NGFI-A and RB following castration suggests that the RB gene may be transcriptionally regulated by NGFI-A in prostate cells.

Original languageEnglish (US)
Pages (from-to)5597-5599
Number of pages3
JournalCancer Research
Volume53
Issue number23
StatePublished - Dec 1993
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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