TY - GEN
T1 - Prostate cancer epithelial mesenchymal transition by TGF-β1 exposure monitored with a photonic crystal biosensor
AU - Cadena, Melissa
AU - Deluna, Frank
AU - Baryeh, Kwaku
AU - Sun, Lu Zhe
AU - Ye, Jing Yong
N1 - Funding Information:
This research was support by the National Institute of Health (NIH) grants R21CA198389, T34 GM060655 and R25GM060655. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the NIH.
Publisher Copyright:
© 2020 SPIE.
PY - 2020
Y1 - 2020
N2 - During prostate cancer progression, cancerous epithelial cells can undergo epithelial-mesenchymal transition (EMT). EMT is a crucial mechanism for the invasion and metastasis of epithelial tumors characterized by the loss of cell-cell adhesion and increased cell mobility. It is associated with biochemical changes such as epithelial cell markers Ecadherin and occludins being down-regulated, and mesenchymal markers vimentin and N-cadherin being upregulated. These changes in protein expression, specifically in the cell membrane, may be monitored via biophysical principles, such as changes in the refractive index (RI) of the cell membrane. In our previous research, we demonstrated the feasibility of using cellular RI as a unique contrast parameter to accomplish label-free detection of prostate cancer cells. In this paper, we report the use of our Photonic-Crystal biosensor in a Total-Internal-Reflection (PC-TIR) configuration to construct a label-free biosensing system, which allows for ultra-sensitive quantification of the changes in cellular RI due to EMT. We induced prostate cancer cells to undergo EMT by exposing these cells to soluble Transforming Growth Factor Beta 1 (TGF-β1). The biophysical characteristics of the cellular RI were quantified extensively in comparison to non-induced cancer cells. Our study shows promising clinical potential in utilizing the PC-TIR biosensing system not only to detect prostate cancer cells, but also to evaluate changes in prostate cancer cells due to EMT.
AB - During prostate cancer progression, cancerous epithelial cells can undergo epithelial-mesenchymal transition (EMT). EMT is a crucial mechanism for the invasion and metastasis of epithelial tumors characterized by the loss of cell-cell adhesion and increased cell mobility. It is associated with biochemical changes such as epithelial cell markers Ecadherin and occludins being down-regulated, and mesenchymal markers vimentin and N-cadherin being upregulated. These changes in protein expression, specifically in the cell membrane, may be monitored via biophysical principles, such as changes in the refractive index (RI) of the cell membrane. In our previous research, we demonstrated the feasibility of using cellular RI as a unique contrast parameter to accomplish label-free detection of prostate cancer cells. In this paper, we report the use of our Photonic-Crystal biosensor in a Total-Internal-Reflection (PC-TIR) configuration to construct a label-free biosensing system, which allows for ultra-sensitive quantification of the changes in cellular RI due to EMT. We induced prostate cancer cells to undergo EMT by exposing these cells to soluble Transforming Growth Factor Beta 1 (TGF-β1). The biophysical characteristics of the cellular RI were quantified extensively in comparison to non-induced cancer cells. Our study shows promising clinical potential in utilizing the PC-TIR biosensing system not only to detect prostate cancer cells, but also to evaluate changes in prostate cancer cells due to EMT.
KW - Cancer Diagnostics
KW - Cellular Refractive Index
KW - Label-Free Biosensor
KW - Optical Biosensor
KW - Photonic Crystal Biosensor
KW - Prostate Cancer
KW - Transforming Growth Factor Beta 1
KW - and Epithelial-mesenchymal transition
UR - http://www.scopus.com/inward/record.url?scp=85082679302&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85082679302&partnerID=8YFLogxK
U2 - 10.1117/12.2544113
DO - 10.1117/12.2544113
M3 - Conference contribution
C2 - 32632340
AN - SCOPUS:85082679302
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Label-Free Biomedical Imaging and Sensing (LBIS) 2020
A2 - Shaked, Natan T.
A2 - Hayden, Oliver
PB - SPIE
T2 - Label-Free Biomedical Imaging and Sensing ,LBIS 2020
Y2 - 1 February 2020 through 4 February 2020
ER -