@inproceedings{14da86e8181e4dfca888673b65517509,
title = "Prostate cancer epithelial mesenchymal transition by TGF-β1 exposure monitored with a photonic crystal biosensor",
abstract = "During prostate cancer progression, cancerous epithelial cells can undergo epithelial-mesenchymal transition (EMT). EMT is a crucial mechanism for the invasion and metastasis of epithelial tumors characterized by the loss of cell-cell adhesion and increased cell mobility. It is associated with biochemical changes such as epithelial cell markers Ecadherin and occludins being down-regulated, and mesenchymal markers vimentin and N-cadherin being upregulated. These changes in protein expression, specifically in the cell membrane, may be monitored via biophysical principles, such as changes in the refractive index (RI) of the cell membrane. In our previous research, we demonstrated the feasibility of using cellular RI as a unique contrast parameter to accomplish label-free detection of prostate cancer cells. In this paper, we report the use of our Photonic-Crystal biosensor in a Total-Internal-Reflection (PC-TIR) configuration to construct a label-free biosensing system, which allows for ultra-sensitive quantification of the changes in cellular RI due to EMT. We induced prostate cancer cells to undergo EMT by exposing these cells to soluble Transforming Growth Factor Beta 1 (TGF-β1). The biophysical characteristics of the cellular RI were quantified extensively in comparison to non-induced cancer cells. Our study shows promising clinical potential in utilizing the PC-TIR biosensing system not only to detect prostate cancer cells, but also to evaluate changes in prostate cancer cells due to EMT.",
keywords = "Cancer Diagnostics, Cellular Refractive Index, Label-Free Biosensor, Optical Biosensor, Photonic Crystal Biosensor, Prostate Cancer, Transforming Growth Factor Beta 1, and Epithelial-mesenchymal transition",
author = "Melissa Cadena and Frank Deluna and Kwaku Baryeh and Sun, {Lu Zhe} and Ye, {Jing Yong}",
note = "Publisher Copyright: {\textcopyright} 2020 SPIE.; Label-Free Biomedical Imaging and Sensing ,LBIS 2020 ; Conference date: 01-02-2020 Through 04-02-2020",
year = "2020",
doi = "10.1117/12.2544113",
language = "English (US)",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
editor = "Shaked, {Natan T.} and Oliver Hayden",
booktitle = "Label-Free Biomedical Imaging and Sensing (LBIS) 2020",
}