TY - JOUR
T1 - Prostate attenuated replication competent adenovirus (ARCA) CN706
T2 - A selective cytotoxic for prostate-specific antigen-positive prostate cancer cells
AU - Rodriguez, Ron
AU - Schuur, Eric R.
AU - Lim, Ho Yeong
AU - Henderson, Gail A.
AU - Simons, Jonathan W.
AU - Henderson, Daniel R.
PY - 1997/7/1
Y1 - 1997/7/1
N2 - Prostate-specific antigen (PSA) is a widely used marker for the diagnosis and management of prostate cancer. Minimal enhancer/promoter constructs derived from the 5' flank of the human PSA gene (prostate-specific enhancer) were inserted into adenovirus type 5 DNA so as to drive the E1A gene, thereby creating a prostate-specific enhancer-containing virus, CN706. E1A was expressed at high levels in CN706-infected human PSA-producing LNCaP cells but not in CN706-infected DU145 cells, which are human prostate cells that do not express PSA. The titer of CN706 was significantly higher in LNCaP cells compared to several human cell lines that do not produce PSA (HBL100, PANC-1, MCF-7, DU145, and OVCAR3). Furthermore, in LNCaP cells, the yield of CN706 was dependent on exogenous androgen (R1881). CN706 destroyed large LNCaP tumors (1 x 109 cells) and abolished PSA production in nu/nu mouse xenograft models with a single intratumoral injection.
AB - Prostate-specific antigen (PSA) is a widely used marker for the diagnosis and management of prostate cancer. Minimal enhancer/promoter constructs derived from the 5' flank of the human PSA gene (prostate-specific enhancer) were inserted into adenovirus type 5 DNA so as to drive the E1A gene, thereby creating a prostate-specific enhancer-containing virus, CN706. E1A was expressed at high levels in CN706-infected human PSA-producing LNCaP cells but not in CN706-infected DU145 cells, which are human prostate cells that do not express PSA. The titer of CN706 was significantly higher in LNCaP cells compared to several human cell lines that do not produce PSA (HBL100, PANC-1, MCF-7, DU145, and OVCAR3). Furthermore, in LNCaP cells, the yield of CN706 was dependent on exogenous androgen (R1881). CN706 destroyed large LNCaP tumors (1 x 109 cells) and abolished PSA production in nu/nu mouse xenograft models with a single intratumoral injection.
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M3 - Article
C2 - 9205053
AN - SCOPUS:0030790684
SN - 0008-5472
VL - 57
SP - 2559
EP - 2563
JO - Cancer Research
JF - Cancer Research
IS - 13
ER -