Prostacyclin increases gastric mucosal blood flow via cyclic AMP

Harold V. Gaskill, Kenneth R Sirinek, Barry A. Levine

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Although prostacyclin has been shown to inhibit stress-induced gastric mucosal ulceration, the mechanism for this cytoprotection is unknown. This experiment measures the effect of intravenous prostacyclin on gastric mucosal blood flow and any modification of this effect by pretreatment with the phosphodiesterase inhibitor, theophylline. Miniature swine were anesthetized, intubated, and ventilated. Three groups of six animals each were studied: (1) theophylline, (2) prostacyclin, and (3) prostacyclin plus theophylline. After stabilization, baseline gastric mucosal blood flow was measured with radioactive spheres. Theophylline (or vehicle) was then infused at 50 mg/kg/hr × 15 min followed by 2 mg/kg/hr thereafter. After 30 min prostacyclin (or vehicle) was infused at 0.005, 0.05, and 0.5 μg/kg/min for three consecutive 15-min periods, each followed by measurement of gastric mucosal blood flow. Results: At 0.5 μg/kg/min, prostacyclin increased gastric mucosal blood flow despite a drop in mean arterial pressure (50.1 ± 8.4 vs 17.4 ± 4.1 ml/100 g/min). Significant differences in cardiac index were not seen among the three groups. Pretreatment with theophylline potentiated prostacyclin's effect on gastric mucosal blood flow suggesting that prostacyclin increases gastric mucosal blood flow by a mechanism dependent on cyclic AMP (113.2 ± 23.3 vs 50.1 ± 8.4 ml/100 g/min). Since mucosal ischemia is an accepted prerequisite for stress-induced ulceration, prostacyclin's effect on blood flow may contribute to its protective effect on gastric mucosa.

Original languageEnglish (US)
Pages (from-to)140-145
Number of pages6
JournalJournal of Surgical Research
Volume33
Issue number2
DOIs
StatePublished - 1982

Fingerprint

Epoprostenol
Cyclic AMP
Stomach
Theophylline
Miniature Swine
Phosphodiesterase Inhibitors
Cytoprotection
Gastric Mucosa
Arterial Pressure
Ischemia

ASJC Scopus subject areas

  • Surgery

Cite this

Prostacyclin increases gastric mucosal blood flow via cyclic AMP. / Gaskill, Harold V.; Sirinek, Kenneth R; Levine, Barry A.

In: Journal of Surgical Research, Vol. 33, No. 2, 1982, p. 140-145.

Research output: Contribution to journalArticle

Gaskill, Harold V. ; Sirinek, Kenneth R ; Levine, Barry A. / Prostacyclin increases gastric mucosal blood flow via cyclic AMP. In: Journal of Surgical Research. 1982 ; Vol. 33, No. 2. pp. 140-145.
@article{bcabfccc997d42e19631639dd228a2e7,
title = "Prostacyclin increases gastric mucosal blood flow via cyclic AMP",
abstract = "Although prostacyclin has been shown to inhibit stress-induced gastric mucosal ulceration, the mechanism for this cytoprotection is unknown. This experiment measures the effect of intravenous prostacyclin on gastric mucosal blood flow and any modification of this effect by pretreatment with the phosphodiesterase inhibitor, theophylline. Miniature swine were anesthetized, intubated, and ventilated. Three groups of six animals each were studied: (1) theophylline, (2) prostacyclin, and (3) prostacyclin plus theophylline. After stabilization, baseline gastric mucosal blood flow was measured with radioactive spheres. Theophylline (or vehicle) was then infused at 50 mg/kg/hr × 15 min followed by 2 mg/kg/hr thereafter. After 30 min prostacyclin (or vehicle) was infused at 0.005, 0.05, and 0.5 μg/kg/min for three consecutive 15-min periods, each followed by measurement of gastric mucosal blood flow. Results: At 0.5 μg/kg/min, prostacyclin increased gastric mucosal blood flow despite a drop in mean arterial pressure (50.1 ± 8.4 vs 17.4 ± 4.1 ml/100 g/min). Significant differences in cardiac index were not seen among the three groups. Pretreatment with theophylline potentiated prostacyclin's effect on gastric mucosal blood flow suggesting that prostacyclin increases gastric mucosal blood flow by a mechanism dependent on cyclic AMP (113.2 ± 23.3 vs 50.1 ± 8.4 ml/100 g/min). Since mucosal ischemia is an accepted prerequisite for stress-induced ulceration, prostacyclin's effect on blood flow may contribute to its protective effect on gastric mucosa.",
author = "Gaskill, {Harold V.} and Sirinek, {Kenneth R} and Levine, {Barry A.}",
year = "1982",
doi = "10.1016/0022-4804(82)90020-8",
language = "English (US)",
volume = "33",
pages = "140--145",
journal = "Journal of Surgical Research",
issn = "0022-4804",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Prostacyclin increases gastric mucosal blood flow via cyclic AMP

AU - Gaskill, Harold V.

AU - Sirinek, Kenneth R

AU - Levine, Barry A.

PY - 1982

Y1 - 1982

N2 - Although prostacyclin has been shown to inhibit stress-induced gastric mucosal ulceration, the mechanism for this cytoprotection is unknown. This experiment measures the effect of intravenous prostacyclin on gastric mucosal blood flow and any modification of this effect by pretreatment with the phosphodiesterase inhibitor, theophylline. Miniature swine were anesthetized, intubated, and ventilated. Three groups of six animals each were studied: (1) theophylline, (2) prostacyclin, and (3) prostacyclin plus theophylline. After stabilization, baseline gastric mucosal blood flow was measured with radioactive spheres. Theophylline (or vehicle) was then infused at 50 mg/kg/hr × 15 min followed by 2 mg/kg/hr thereafter. After 30 min prostacyclin (or vehicle) was infused at 0.005, 0.05, and 0.5 μg/kg/min for three consecutive 15-min periods, each followed by measurement of gastric mucosal blood flow. Results: At 0.5 μg/kg/min, prostacyclin increased gastric mucosal blood flow despite a drop in mean arterial pressure (50.1 ± 8.4 vs 17.4 ± 4.1 ml/100 g/min). Significant differences in cardiac index were not seen among the three groups. Pretreatment with theophylline potentiated prostacyclin's effect on gastric mucosal blood flow suggesting that prostacyclin increases gastric mucosal blood flow by a mechanism dependent on cyclic AMP (113.2 ± 23.3 vs 50.1 ± 8.4 ml/100 g/min). Since mucosal ischemia is an accepted prerequisite for stress-induced ulceration, prostacyclin's effect on blood flow may contribute to its protective effect on gastric mucosa.

AB - Although prostacyclin has been shown to inhibit stress-induced gastric mucosal ulceration, the mechanism for this cytoprotection is unknown. This experiment measures the effect of intravenous prostacyclin on gastric mucosal blood flow and any modification of this effect by pretreatment with the phosphodiesterase inhibitor, theophylline. Miniature swine were anesthetized, intubated, and ventilated. Three groups of six animals each were studied: (1) theophylline, (2) prostacyclin, and (3) prostacyclin plus theophylline. After stabilization, baseline gastric mucosal blood flow was measured with radioactive spheres. Theophylline (or vehicle) was then infused at 50 mg/kg/hr × 15 min followed by 2 mg/kg/hr thereafter. After 30 min prostacyclin (or vehicle) was infused at 0.005, 0.05, and 0.5 μg/kg/min for three consecutive 15-min periods, each followed by measurement of gastric mucosal blood flow. Results: At 0.5 μg/kg/min, prostacyclin increased gastric mucosal blood flow despite a drop in mean arterial pressure (50.1 ± 8.4 vs 17.4 ± 4.1 ml/100 g/min). Significant differences in cardiac index were not seen among the three groups. Pretreatment with theophylline potentiated prostacyclin's effect on gastric mucosal blood flow suggesting that prostacyclin increases gastric mucosal blood flow by a mechanism dependent on cyclic AMP (113.2 ± 23.3 vs 50.1 ± 8.4 ml/100 g/min). Since mucosal ischemia is an accepted prerequisite for stress-induced ulceration, prostacyclin's effect on blood flow may contribute to its protective effect on gastric mucosa.

UR - http://www.scopus.com/inward/record.url?scp=0019984374&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019984374&partnerID=8YFLogxK

U2 - 10.1016/0022-4804(82)90020-8

DO - 10.1016/0022-4804(82)90020-8

M3 - Article

C2 - 6285082

AN - SCOPUS:0019984374

VL - 33

SP - 140

EP - 145

JO - Journal of Surgical Research

JF - Journal of Surgical Research

SN - 0022-4804

IS - 2

ER -