Prophylactic effect of melatonin in reducing lead-induced neurotoxicity in the rat

Gamal H. El-Sokkary, Esam S. Kamel, Russel J Reiter

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Oxidative stress is a likely molecular mechanism in lead neurotoxicity. Considering the antioxidant properties of melatonin, this study investigated the neuroprotective potential of melatonin in the hippocampus and corpus striatum of rats treated with lead. Three groups of male rats (control, lead acetate-treated [100 mg/kg], and lead acetate plus melatonin [10 mg/kg] for 21 consecutive days) were used. Levels of products of lipid peroxidation (LPO), glutathione (GSH) and superoxide dismutase (SOD) activity were measured in brain homogenates. Histological changes in the pyramidal cells of the hippocampus and the putamen of the corpus striatum were examined. The results documented increased LPO and decreased GSH and SOD activity in the brain homogenates of lead-treated rats. Histological observations revealed severe damage and a reduction in neuronal density in the hippocampus and corpus striatum. When melatonin was given to lead-treated rats, it almost completely attenuated the increase in LPO products and restored GSH levels and SOD activity. Also, the morphological damage was reduced and neuronal density was restored by melatonin. Considering the ease with which melatonin enters the brain, these results, along with previous observations, suggest that melatonin may be useful in combating free radical-induced neuronal injury that is a result of lead toxicity.

Original languageEnglish (US)
Pages (from-to)461-470
Number of pages10
JournalCellular and Molecular Biology Letters
Volume8
Issue number2
StatePublished - 2003

Fingerprint

Melatonin
Rats
Corpus Striatum
Lipid Peroxidation
Superoxide Dismutase
Hippocampus
Brain
Lipids
Rat control
Oxidative stress
Pyramidal Cells
Putamen
Lead
Free Radicals
Glutathione
Toxicity
Oxidative Stress
Antioxidants
Wounds and Injuries

Keywords

  • Corpus Striatum
  • Glutathione
  • Hippocampus
  • Lead Acetate
  • Lipid Peroxidation
  • Melatonin
  • Superoxide Dismutase

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Prophylactic effect of melatonin in reducing lead-induced neurotoxicity in the rat. / El-Sokkary, Gamal H.; Kamel, Esam S.; Reiter, Russel J.

In: Cellular and Molecular Biology Letters, Vol. 8, No. 2, 2003, p. 461-470.

Research output: Contribution to journalArticle

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