Promotion of Rad51-dependent D-loop formation by yeast recombination factor Rdh54/Tid1

Galina Petukhova, Patrick Sung, Hannah Klein

Research output: Contribution to journalArticle

103 Scopus citations

Abstract

The first DNA joint formed in homologous recombination processes is a D-loop. Saccharomyces cerevisiae RDH54/TID1-encoded product, a Swi2/Snf2-like factor involved in recombination, is shown here to promote D-loop formation with Rad51 recombinase. Physical interaction between Rdh54 and Rad51 is functionally important because Rdh54 does not enhance the recombinase activity of the Escherichia coli RecA protein. Robust dsDNA-activated ATPase activity in Rdh54 generates unconstrained negative and positive supercoils in DNA. Efficient D-loop formation occurs with even topologically relaxed DNA, suggesting that via specific protein-protein interactions, the negative supercoils produced by Rdh54 are used by Rad51 for making DNA joints.

Original languageEnglish (US)
Pages (from-to)2206-2215
Number of pages10
JournalGenes and Development
Volume14
Issue number17
DOIs
StatePublished - 2000

Keywords

  • DNA double-strand break repair
  • DNA supercoiling
  • Homologous recombination
  • Meiosis

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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