Proline- and arginine-rich peptides constitute a novel class of allosteric inhibitors of proteasome activity

Maria Gaczynska, Pawel A. Osmulski, Youhe Gao, Mark J. Post, Michael Simons

Research output: Contribution to journalArticle

103 Scopus citations

Abstract

Substrate-specific inhibition of the proteasome has been unachievable despite great interest in proteasome inhibitors as drugs. Recent studies demonstrated that PR39, a natural proline- and arginine-rich antibacterial peptide, stimulates angiogenesis and inhibits inflammatory responses by specifically blocking degradation of IκBa and HIF-1α by the proteasome. However, molecular events involved in the PR39 - proteasome interaction have not been elucidated. Here we show that PR39 is a noncompetitive and reversible inhibitor of the proteasome function. This effect is achieved by a unique allosteric mechanism allowing for specific inhibition of degradation of selected proteins without affecting total proteasome-dependent proteolysis. Atomic force microscopy (AFM) studies demonstrate that 20S and 26S proteasomes treated with PR39 or its derivatives exhibit serious perturbations in their structure and their normal allosteric movements. These effects are universal for proteasomes from yeast to human. The shortest functional sequence derived from PR39 still showing the allosteric inhibitory effect consists of eleven NH2-terminal residues containing essential three NH2-terminal arginines. The noncompetitive and reversible in vitro action of PR39 and its truncated derivatives is matched by the ability of the peptides to induce angiogenesis in vivo. We postulate that PR39 changes conformational dynamics of the proteasomes by interactions with the noncatalytic subunit α7 in a way that prevents the enzyme from cleaving the substrates of unique structural constraints.

Original languageEnglish (US)
Pages (from-to)8663-8670
Number of pages8
JournalBiochemistry
Volume42
Issue number29
DOIs
Publication statusPublished - Jul 29 2003

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ASJC Scopus subject areas

  • Biochemistry

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