TY - JOUR
T1 - Prognostic value of an RNA expression signature derived from cell cycle proliferation genes in patients with prostate cancer
T2 - A retrospective study
AU - Cuzick, Jack
AU - Swanson, Gregory P.
AU - Fisher, Gabrielle
AU - Brothman, Arthur R.
AU - Berney, Daniel M.
AU - Reid, Julia E.
AU - Mesher, David
AU - Speights, V. O.
AU - Stankiewicz, Elzbieta
AU - Foster, Christopher S.
AU - Møller, Henrik
AU - Scardino, Peter
AU - Warren, Jorja D.
AU - Park, Jimmy
AU - Younus, Adib
AU - Flake, Darl D.
AU - Wagner, Susanne
AU - Gutin, Alexander
AU - Lanchbury, Jerry S.
AU - Stone, Steven
N1 - Funding Information:
We gratefully recognise the patients who participated in this study and the support from Cancer Research UK, Queen Mary University of London, the Orchid Appeal, National Institutes of Health ( SPORE CA92629 ), and the Koch Foundation and Myriad Genetics. We also thank investigators and staff in the cancer registries and participating hospitals for their support ( webappendix p 6 ).
PY - 2011/3
Y1 - 2011/3
N2 - Background: Optimum management of clinically localised prostate cancer presents unique challenges because of the highly variable and often indolent natural history of the disease. To predict disease aggressiveness, clinicians combine clinical variables to create prognostic models, but the models have limited accuracy. We assessed the prognostic value of a predefined cell cycle progression (CCP) score in two cohorts of patients with prostate cancer. Methods: We measured the expression of 31 genes involved in CCP with quantitative RT-PCR on RNA extracted from formalin-fixed paraffin-embedded tumour samples, and created a predefined score and assessed its usefulness in the prediction of disease outcome. The signature was assessed retrospectively in a cohort of patients from the USA who had undergone radical prostatectomy, and in a cohort of randomly selected men with clinically localised prostate cancer diagnosed by use of a transurethral resection of the prostate (TURP) in the UK who were managed conservatively. The primary endpoint was time to biochemical recurrence for the cohort of patients who had radical prostatectomy, and time to death from prostate cancer for the TURP cohort. Findings: After prostatectomy, the CCP score was useful for predicting biochemical recurrence in the univariate analysis (hazard ratio for a 1-unit change [doubling] in CCP 1·89; 95% CI 1·54-2·31; p=5·6×10-9) and the best multivariate analysis (1·77, 1·40-2·22; p=4·3×10-6). In the best predictive model (final multivariate analysis), the CCP score and prostate-specific antigen (PSA) concentration were the most important variables and were more significant than any other clinical variable. In the TURP cohort, the CCP score was the most important variable for prediction of time to death from prostate cancer in both univariate analysis (2·92, 2·38-3·57, p=6·1×10-22) and the final multivariate analysis (2·57, 1·93-3·43; p=8·2×10-11), and was stronger than all other prognostic factors, although PSA concentration also added useful information. Heterogeneity in the hazard ratio for the CCP score was not noted in any case for any clinical variables. Interpretation: The results of this study provide strong evidence that the CCP score is a robust prognostic marker, which, after additional validation, could have an essential role in determining the appropriate treatment for patients with prostate cancer. Funding: Cancer Research UK, Queen Mary University of London, Orchid Appeal, US National Institutes of Health, and Koch Foundation.
AB - Background: Optimum management of clinically localised prostate cancer presents unique challenges because of the highly variable and often indolent natural history of the disease. To predict disease aggressiveness, clinicians combine clinical variables to create prognostic models, but the models have limited accuracy. We assessed the prognostic value of a predefined cell cycle progression (CCP) score in two cohorts of patients with prostate cancer. Methods: We measured the expression of 31 genes involved in CCP with quantitative RT-PCR on RNA extracted from formalin-fixed paraffin-embedded tumour samples, and created a predefined score and assessed its usefulness in the prediction of disease outcome. The signature was assessed retrospectively in a cohort of patients from the USA who had undergone radical prostatectomy, and in a cohort of randomly selected men with clinically localised prostate cancer diagnosed by use of a transurethral resection of the prostate (TURP) in the UK who were managed conservatively. The primary endpoint was time to biochemical recurrence for the cohort of patients who had radical prostatectomy, and time to death from prostate cancer for the TURP cohort. Findings: After prostatectomy, the CCP score was useful for predicting biochemical recurrence in the univariate analysis (hazard ratio for a 1-unit change [doubling] in CCP 1·89; 95% CI 1·54-2·31; p=5·6×10-9) and the best multivariate analysis (1·77, 1·40-2·22; p=4·3×10-6). In the best predictive model (final multivariate analysis), the CCP score and prostate-specific antigen (PSA) concentration were the most important variables and were more significant than any other clinical variable. In the TURP cohort, the CCP score was the most important variable for prediction of time to death from prostate cancer in both univariate analysis (2·92, 2·38-3·57, p=6·1×10-22) and the final multivariate analysis (2·57, 1·93-3·43; p=8·2×10-11), and was stronger than all other prognostic factors, although PSA concentration also added useful information. Heterogeneity in the hazard ratio for the CCP score was not noted in any case for any clinical variables. Interpretation: The results of this study provide strong evidence that the CCP score is a robust prognostic marker, which, after additional validation, could have an essential role in determining the appropriate treatment for patients with prostate cancer. Funding: Cancer Research UK, Queen Mary University of London, Orchid Appeal, US National Institutes of Health, and Koch Foundation.
UR - http://www.scopus.com/inward/record.url?scp=79952042778&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79952042778&partnerID=8YFLogxK
U2 - 10.1016/S1470-2045(10)70295-3
DO - 10.1016/S1470-2045(10)70295-3
M3 - Article
C2 - 21310658
AN - SCOPUS:79952042778
VL - 12
SP - 245
EP - 255
JO - The Lancet Oncology
JF - The Lancet Oncology
SN - 1470-2045
IS - 3
ER -