Prognostic significance of CD38 and CD20 expression as assessed by quantitative flow cytometry in chronic lymphocytic leukaemia

Eric D. Hsi, Kenneth J. Kopecky, Frederick R. Appelbaum, David Boldt, Tom Frey, Margaret Loftus, Mohamad A. Hussein

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

CD38 expression on chronic lymphocytic leukaemia (CLL) cells is a poor prognostic factor, however, methods for measuring this vary. The QuantiBRITE™ flow cytometry (FC) system yields an absolute antigen expression value (antibodies bound/cell, ABC) and may be useful in standardizing CD38 expression analysis. We evaluated cryopreserved pretreatment CLL cells for CD20 ABC, CD38 ABC, and percentage of CD38+ B cells from 131 patients requiring therapy. The 92 patients (70%) with ≥ 100 CD38 ABC had worse overall survival (OS; 34% at 5 years) compared with those with <100 CD38 ABC (70% at 5 years, mortality hazard ratio 2.30, 95% confidence interval 1.28-4.12; two-tailed P = 0.003). Among the 64 patients with <30% CD38+ cells. OS of the 25 with ≥ 100 ABC was worse than that of the 39 with < 100 ABC (P = 0.018). OS of patients with < 30% CD38+ cells and ≥ 100 ABC was actually similar to that of patients with ≥ 30% CD38+ cells. BrightCD20 expression (≥ 20 000 ABC) was not associated with a worse as (P = 0.10). The presence of ≥ 100 CD38 ABC in CLL patients requiring therapy is an unfavourable prognostic factor for as and quantitative FC may be superior to percentage CD38+ cell assessment. Prospective trials are required to determine more precisely the prognostic significance of absolute expression levels in fresh CLL cells.

Original languageEnglish (US)
Pages (from-to)1017-1025
Number of pages9
JournalBritish Journal of Haematology
Volume120
Issue number6
DOIs
StatePublished - Mar 2003

Keywords

  • CD38
  • CLL
  • Flow cytometry
  • Prognosis
  • Quantification

ASJC Scopus subject areas

  • Hematology

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