Profiling the Anaerobic Response of C. elegans Using GC-MS

Jeffrey A. Butler, Robert J. Mishur, Alex F. Bokov, Kevin W. Hakala, Susan E Weintraub, Shane L. Rea

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The nematode Caenorhabditis elegans is a model organism that has seen extensive use over the last four decades in multiple areas of investigation. In this study we explore the response of the nematode Caenorhabditis elegans to acute anoxia using gas-chromatography mass-spectrometry (GC-MS). We focus on the readily-accessible worm exometabolome to show that C. elegans are mixed acid fermenters that utilize several metabolic pathways in unconventional ways to remove reducing equivalents - including partial reversal of branched-chain amino acid catabolism and a potentially novel use of the glyoxylate pathway. In doing so, we provide detailed methods for the collection and analysis of excreted metabolites that, with minimal adjustment, should be applicable to many other species. We also describe a procedure for collecting highly volatile compounds from C. elegans. We are distributing our mass spectral library in an effort to facilitate wider use of metabolomics.

Original languageEnglish (US)
Article numbere46140
JournalPLoS One
Volume7
Issue number9
DOIs
StatePublished - Sep 27 2012

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Fermenters
Branched Chain Amino Acids
Caenorhabditis elegans
Metabolites
Gas chromatography
Gas Chromatography-Mass Spectrometry
Mass spectrometry
Acids
Nematoda
branched chain amino acids
Metabolomics
fermenters
amino acid metabolism
metabolomics
Metabolic Networks and Pathways
volatile compounds
Libraries
biochemical pathways
hypoxia
metabolites

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Butler, J. A., Mishur, R. J., Bokov, A. F., Hakala, K. W., Weintraub, S. E., & Rea, S. L. (2012). Profiling the Anaerobic Response of C. elegans Using GC-MS. PLoS One, 7(9), [e46140]. https://doi.org/10.1371/journal.pone.0046140

Profiling the Anaerobic Response of C. elegans Using GC-MS. / Butler, Jeffrey A.; Mishur, Robert J.; Bokov, Alex F.; Hakala, Kevin W.; Weintraub, Susan E; Rea, Shane L.

In: PLoS One, Vol. 7, No. 9, e46140, 27.09.2012.

Research output: Contribution to journalArticle

Butler, JA, Mishur, RJ, Bokov, AF, Hakala, KW, Weintraub, SE & Rea, SL 2012, 'Profiling the Anaerobic Response of C. elegans Using GC-MS', PLoS One, vol. 7, no. 9, e46140. https://doi.org/10.1371/journal.pone.0046140
Butler, Jeffrey A. ; Mishur, Robert J. ; Bokov, Alex F. ; Hakala, Kevin W. ; Weintraub, Susan E ; Rea, Shane L. / Profiling the Anaerobic Response of C. elegans Using GC-MS. In: PLoS One. 2012 ; Vol. 7, No. 9.
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