Profiling of human antibody responses to Chlamydia trachomatis urogenital tract infection using microplates arrayed with 156 chlamydial fusion proteins

Jyotika Sharma, Youmin Zhong, Feng Dong, Jeanna M. Piper, Guqi Wang, Guangming Zhong

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

The available chlamydial genome sequences have made it possible to comprehensively analyze host responses to all chlamydial proteins, which is essential for further understanding of chlamydial pathogenesis and development of effective chlamydial vaccines. Microplates arrayed with 156 Chlamydia trachomatis fusion proteins were used to evaluate antibody responses in women urogenitally infected with C. trachomatis. Based on both the antibody recognition frequency and iiter, seven chlamydial antigens encoded by open reading frames (ORFs) CT089, CT147, CT226, CT681, CT694, CT795, and CT858, respectively, were identified as relatively immunodominant; six of these are encoded by hypothetical ORFs. Antibody binding to these chlamydial fusion proteins was blocked by C. trachomatis-infected but not by normal HeLa cell lysates or irrelevant bacterial lysates. These results have revealed novel immune-reactive chlamydial antigens, not only indicating that the hypothetical ORF-encoded proteins are expressed during chlamydial infection in humans but also providing the proof of principle that the fusion protein-based approach can be used to profile human immune responses to chlamydial infection at the whole-genome scale.

Original languageEnglish (US)
Pages (from-to)1490-1499
Number of pages10
JournalInfection and Immunity
Volume74
Issue number3
DOIs
StatePublished - Mar 2006

Fingerprint

Chlamydia trachomatis
Antibody Formation
Open Reading Frames
Infection
Proteins
Genome
Antigens
Antibodies
HeLa Cells
Vaccines

ASJC Scopus subject areas

  • Immunology

Cite this

Profiling of human antibody responses to Chlamydia trachomatis urogenital tract infection using microplates arrayed with 156 chlamydial fusion proteins. / Sharma, Jyotika; Zhong, Youmin; Dong, Feng; Piper, Jeanna M.; Wang, Guqi; Zhong, Guangming.

In: Infection and Immunity, Vol. 74, No. 3, 03.2006, p. 1490-1499.

Research output: Contribution to journalArticle

@article{544857543ca24bda863db663d97450be,
title = "Profiling of human antibody responses to Chlamydia trachomatis urogenital tract infection using microplates arrayed with 156 chlamydial fusion proteins",
abstract = "The available chlamydial genome sequences have made it possible to comprehensively analyze host responses to all chlamydial proteins, which is essential for further understanding of chlamydial pathogenesis and development of effective chlamydial vaccines. Microplates arrayed with 156 Chlamydia trachomatis fusion proteins were used to evaluate antibody responses in women urogenitally infected with C. trachomatis. Based on both the antibody recognition frequency and iiter, seven chlamydial antigens encoded by open reading frames (ORFs) CT089, CT147, CT226, CT681, CT694, CT795, and CT858, respectively, were identified as relatively immunodominant; six of these are encoded by hypothetical ORFs. Antibody binding to these chlamydial fusion proteins was blocked by C. trachomatis-infected but not by normal HeLa cell lysates or irrelevant bacterial lysates. These results have revealed novel immune-reactive chlamydial antigens, not only indicating that the hypothetical ORF-encoded proteins are expressed during chlamydial infection in humans but also providing the proof of principle that the fusion protein-based approach can be used to profile human immune responses to chlamydial infection at the whole-genome scale.",
author = "Jyotika Sharma and Youmin Zhong and Feng Dong and Piper, {Jeanna M.} and Guqi Wang and Guangming Zhong",
year = "2006",
month = "3",
doi = "10.1128/IAI.74.3.1490-1499.2006",
language = "English (US)",
volume = "74",
pages = "1490--1499",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "3",

}

TY - JOUR

T1 - Profiling of human antibody responses to Chlamydia trachomatis urogenital tract infection using microplates arrayed with 156 chlamydial fusion proteins

AU - Sharma, Jyotika

AU - Zhong, Youmin

AU - Dong, Feng

AU - Piper, Jeanna M.

AU - Wang, Guqi

AU - Zhong, Guangming

PY - 2006/3

Y1 - 2006/3

N2 - The available chlamydial genome sequences have made it possible to comprehensively analyze host responses to all chlamydial proteins, which is essential for further understanding of chlamydial pathogenesis and development of effective chlamydial vaccines. Microplates arrayed with 156 Chlamydia trachomatis fusion proteins were used to evaluate antibody responses in women urogenitally infected with C. trachomatis. Based on both the antibody recognition frequency and iiter, seven chlamydial antigens encoded by open reading frames (ORFs) CT089, CT147, CT226, CT681, CT694, CT795, and CT858, respectively, were identified as relatively immunodominant; six of these are encoded by hypothetical ORFs. Antibody binding to these chlamydial fusion proteins was blocked by C. trachomatis-infected but not by normal HeLa cell lysates or irrelevant bacterial lysates. These results have revealed novel immune-reactive chlamydial antigens, not only indicating that the hypothetical ORF-encoded proteins are expressed during chlamydial infection in humans but also providing the proof of principle that the fusion protein-based approach can be used to profile human immune responses to chlamydial infection at the whole-genome scale.

AB - The available chlamydial genome sequences have made it possible to comprehensively analyze host responses to all chlamydial proteins, which is essential for further understanding of chlamydial pathogenesis and development of effective chlamydial vaccines. Microplates arrayed with 156 Chlamydia trachomatis fusion proteins were used to evaluate antibody responses in women urogenitally infected with C. trachomatis. Based on both the antibody recognition frequency and iiter, seven chlamydial antigens encoded by open reading frames (ORFs) CT089, CT147, CT226, CT681, CT694, CT795, and CT858, respectively, were identified as relatively immunodominant; six of these are encoded by hypothetical ORFs. Antibody binding to these chlamydial fusion proteins was blocked by C. trachomatis-infected but not by normal HeLa cell lysates or irrelevant bacterial lysates. These results have revealed novel immune-reactive chlamydial antigens, not only indicating that the hypothetical ORF-encoded proteins are expressed during chlamydial infection in humans but also providing the proof of principle that the fusion protein-based approach can be used to profile human immune responses to chlamydial infection at the whole-genome scale.

UR - http://www.scopus.com/inward/record.url?scp=33644764765&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33644764765&partnerID=8YFLogxK

U2 - 10.1128/IAI.74.3.1490-1499.2006

DO - 10.1128/IAI.74.3.1490-1499.2006

M3 - Article

C2 - 16495519

AN - SCOPUS:33644764765

VL - 74

SP - 1490

EP - 1499

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 3

ER -