Profiling DNA methylomes from microarray to genome-scale sequencing

Yi Wen Huang, Tim H.M. Huang, Li Shu Wang

Research output: Contribution to journalReview articlepeer-review

45 Scopus citations

Abstract

DNA cytosine methylation is a central epigenetic modi.cation which plays critical roles in cellular processes including genome regulation, development and disease. Here, we review current and emerging microarray and next-generation sequencing based technologies that enhance our knowledge of DNA methylation pro.ling. Each methodology has limitations and their unique applications, and combinations of several modalities may help build the entire methylome. With advances on next-generation sequencing technologies, it is now possible to globally map the DNA cytosine methylation at single-base resolution, providing new insights into the regulation and dynamics of DNA methylation in genomes.

Original languageEnglish (US)
Pages (from-to)139-147
Number of pages9
JournalTechnology in Cancer Research and Treatment
Volume9
Issue number2
DOIs
StatePublished - Apr 2010

Keywords

  • DNA methylation
  • Microarray
  • Next-generation sequencing

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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