TY - JOUR
T1 - Profiling and relative quantification of phosphatidylethanolamine based on acetone stable isotope derivatization
AU - Wang, Xiang
AU - Wei, Fang
AU - Xu, Ji qu
AU - Lv, Xin
AU - Dong, Xu yan
AU - Han, Xianlin
AU - Quek, Siew young
AU - Huang, Feng hong
AU - Chen, Hong
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China (Grant No. 31571926 and 31271879 ) and the National High Technology Research and Development Program of China (863 Program: 2011AA100904 ). We also gratefully acknowledge the support of Hubei Natural Science Foundation (Grant No. 2013CFB425 ), Hubei International Cooperation Project (Grant No. 2015BHE017 ) and Chinese Academy of Agricultural Innovation Project . The authors also thank the support from Lipid Chemistry and Nutrition Joint Lab between Institute of Oil Crops Research, Chinese Academy of Agricultural Sciences and Faculty of Science, University of Auckland .
Publisher Copyright:
© 2015 Elsevier B.V.
PY - 2016/1/1
Y1 - 2016/1/1
N2 - Phosphatidylethanolamine (PE) is considered to be one of the pivotal lipids for normal cellular function as well as disease initiation and progression. In this study, a simple, efficient, reliable, and inexpensive method for the qualitative analysis and relative quantification of PE, based on acetone stable isotope derivatization combined with double neutral loss scan-shotgun electrospray ionization tandem-quadrupole mass spectrometry analysis (ASID-DNLS-Shotgun ESI-MS/MS), was developed. The ASID method led to alkylation of the primary amino groups of PE with an isopropyl moiety. The use of acetone (d0-acetone) and deuterium-labeled acetone (d6-acetone) introduced a 6 Da mass shift that was ideally suited for relative quantitative analysis, and enhanced sensitivity for mass analysis. The DNLS model was introduced to simultaneously analyze the differential derivatized PEs by shotgun ESI-MS/MS with high selectivity and accuracy. The reaction specificity, labeling efficiency, and linearity of the ASID method were thoroughly evaluated in this study. Its excellent applicability was validated by qualitative and relative quantitative analysis of PE species presented in liver samples from rats fed different diets. Using the ASID-DNLS-Shotgun ESI-MS/MS method, 45 PE species from rat livers have been identified and quantified in an efficient manner. The level of total PEs tended to decrease in the livers of rats on high fat diets compared with controls. The levels of PE 32:1, 34:3, 34:2, 36:3, 36:2, 42:10, plasmalogen PE 36:1 and lyso PE 22:6 were significantly reduced, while levels of PE 36:1 and lyso PE 16:0 increased.
AB - Phosphatidylethanolamine (PE) is considered to be one of the pivotal lipids for normal cellular function as well as disease initiation and progression. In this study, a simple, efficient, reliable, and inexpensive method for the qualitative analysis and relative quantification of PE, based on acetone stable isotope derivatization combined with double neutral loss scan-shotgun electrospray ionization tandem-quadrupole mass spectrometry analysis (ASID-DNLS-Shotgun ESI-MS/MS), was developed. The ASID method led to alkylation of the primary amino groups of PE with an isopropyl moiety. The use of acetone (d0-acetone) and deuterium-labeled acetone (d6-acetone) introduced a 6 Da mass shift that was ideally suited for relative quantitative analysis, and enhanced sensitivity for mass analysis. The DNLS model was introduced to simultaneously analyze the differential derivatized PEs by shotgun ESI-MS/MS with high selectivity and accuracy. The reaction specificity, labeling efficiency, and linearity of the ASID method were thoroughly evaluated in this study. Its excellent applicability was validated by qualitative and relative quantitative analysis of PE species presented in liver samples from rats fed different diets. Using the ASID-DNLS-Shotgun ESI-MS/MS method, 45 PE species from rat livers have been identified and quantified in an efficient manner. The level of total PEs tended to decrease in the livers of rats on high fat diets compared with controls. The levels of PE 32:1, 34:3, 34:2, 36:3, 36:2, 42:10, plasmalogen PE 36:1 and lyso PE 22:6 were significantly reduced, while levels of PE 36:1 and lyso PE 16:0 increased.
KW - Acetone stable isotope derivatization
KW - Mass spectrometry
KW - Phosphatidylethanolamine
KW - Relative quantification
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U2 - 10.1016/j.aca.2015.11.003
DO - 10.1016/j.aca.2015.11.003
M3 - Article
C2 - 26703264
AN - SCOPUS:84956941576
VL - 902
SP - 142
EP - 153
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
SN - 0003-2670
ER -