Abstract
Primary baboon hepatocytes were cultured in a serum-free medium formulation that permitted the analysis of lipoprotein(a) (Lp(a)) production by the cells. The hepatocytes were determined to synthesize Lp(a) on the basis of the following observations: (1) the culture medium reacted in an ELISA designed for detection of baboon Lp(a) in serum samples; (2) the Lp(a)-specific protein, apo(a), was detected in the culture medium by immunoblotting techniques; (3) the unique protein structure of Lp(a) was demonstrated (i.e., association of apo(a) with apoB via interchain disulfide bonds to form apoLp(a)); and (4) the Lp(a) proteins occurred in the medium at a density of about 1.05 g/ml when subjected to density gradient ultracentrifugation. De novo synthesis of Lp(a) by cultured hepatocytes was demonstrated by incorporation of [35S]cysteine. Lp(a) was produced by the hepatocytes throughout a 20 day culture period. Finally, apo(a) isoform patterns in the hepatocyte culture medium and the hepatocyte donors' serum were indistinguishable.
Original language | English (US) |
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Pages (from-to) | 30-35 |
Number of pages | 6 |
Journal | Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism |
Volume | 1003 |
Issue number | 1 |
DOIs | |
State | Published - May 15 1989 |
Keywords
- (Baboon)
- Apolipoprotein a
- ApolipoproteinB
- Lipoprotein(a)
- Primary hepatocyte
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Endocrinology