Production of lipoprotein(a) by primary baboon hepatocytes

David L. Rainwater, Robert E. Lanford

    Research output: Contribution to journalArticlepeer-review

    43 Scopus citations

    Abstract

    Primary baboon hepatocytes were cultured in a serum-free medium formulation that permitted the analysis of lipoprotein(a) (Lp(a)) production by the cells. The hepatocytes were determined to synthesize Lp(a) on the basis of the following observations: (1) the culture medium reacted in an ELISA designed for detection of baboon Lp(a) in serum samples; (2) the Lp(a)-specific protein, apo(a), was detected in the culture medium by immunoblotting techniques; (3) the unique protein structure of Lp(a) was demonstrated (i.e., association of apo(a) with apoB via interchain disulfide bonds to form apoLp(a)); and (4) the Lp(a) proteins occurred in the medium at a density of about 1.05 g/ml when subjected to density gradient ultracentrifugation. De novo synthesis of Lp(a) by cultured hepatocytes was demonstrated by incorporation of [35S]cysteine. Lp(a) was produced by the hepatocytes throughout a 20 day culture period. Finally, apo(a) isoform patterns in the hepatocyte culture medium and the hepatocyte donors' serum were indistinguishable.

    Original languageEnglish (US)
    Pages (from-to)30-35
    Number of pages6
    JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
    Volume1003
    Issue number1
    DOIs
    StatePublished - May 15 1989

    Keywords

    • (Baboon)
    • Apolipoprotein a
    • ApolipoproteinB
    • Lipoprotein(a)
    • Primary hepatocyte

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Endocrinology

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