Production of functional S-II in Bombyx mori cells

Shoji Hirashima; Nobuo Horikoshi; Kazuhisa Sekimizu; Shunji Natori

doi:10.1016/S0006-291X(89)80115-9

Production of functional S-II in Bombyx mori cells

Shoji Hirashima
, Nobuo Horikoshi
, Kazuhisa Sekimizu
, Shunji Natori

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A cDNA for murine transcription factor S-II (Hirashima et al., J. Biol. Chem (1988) 263, 3858-3863) was inserted into a silkworm baculovirus vector and expressed in Bm-N cells, a cell line of Bombyx mori maintained in the laboratory. Recombinant S-II was purified from virus-infected cell extracts to near homogeneity by ammonium sulfate fractionation, and chromatographies on DEAE-cellulose and phosphocellulose. About 60 μg of recombinant S-II was obtained from 1 g of virus-infected cells. The molecular mass and specific activity of recombinant S-II were exactly the same as those of authentic S-II purified from Ehrlich ascites tumor cells.

Original language	English (US)
Pages (from-to)	1093-1099
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	160
Issue number	3
DOIs	https://doi.org/10.1016/S0006-291X(89)80115-9
State	Published - May 15 1989
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/S0006-291X(89)80115-9

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@article{dfa903cb48da4a52bc78a6c2243ddf38,

title = "Production of functional S-II in Bombyx mori cells",

abstract = "A cDNA for murine transcription factor S-II (Hirashima et al., J. Biol. Chem (1988) 263, 3858-3863) was inserted into a silkworm baculovirus vector and expressed in Bm-N cells, a cell line of Bombyx mori maintained in the laboratory. Recombinant S-II was purified from virus-infected cell extracts to near homogeneity by ammonium sulfate fractionation, and chromatographies on DEAE-cellulose and phosphocellulose. About 60 μg of recombinant S-II was obtained from 1 g of virus-infected cells. The molecular mass and specific activity of recombinant S-II were exactly the same as those of authentic S-II purified from Ehrlich ascites tumor cells.",

author = "Shoji Hirashima and Nobuo Horikoshi and Kazuhisa Sekimizu and Shunji Natori",

note = "Funding Information: We wish to thank Drs. Y. Saeki and M. Furusawa (Daiichi Seiyaku Co. Ltd.) for providing the baculovirus expression system and for helping us to construct recombinant viruses. This study was supported by a Grant-in-Aid for Scientific Research on Priority Areas of Transcription Factors from the Ministry of Education, Science and Culture of Japan and a grant from the Japan Health Science Foundation.",

year = "1989",

month = may,

day = "15",

doi = "10.1016/S0006-291X(89)80115-9",

language = "English (US)",

volume = "160",

pages = "1093--1099",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "3",

}

TY - JOUR

T1 - Production of functional S-II in Bombyx mori cells

AU - Hirashima, Shoji

AU - Horikoshi, Nobuo

AU - Sekimizu, Kazuhisa

AU - Natori, Shunji

N1 - Funding Information: We wish to thank Drs. Y. Saeki and M. Furusawa (Daiichi Seiyaku Co. Ltd.) for providing the baculovirus expression system and for helping us to construct recombinant viruses. This study was supported by a Grant-in-Aid for Scientific Research on Priority Areas of Transcription Factors from the Ministry of Education, Science and Culture of Japan and a grant from the Japan Health Science Foundation.

PY - 1989/5/15

Y1 - 1989/5/15

N2 - A cDNA for murine transcription factor S-II (Hirashima et al., J. Biol. Chem (1988) 263, 3858-3863) was inserted into a silkworm baculovirus vector and expressed in Bm-N cells, a cell line of Bombyx mori maintained in the laboratory. Recombinant S-II was purified from virus-infected cell extracts to near homogeneity by ammonium sulfate fractionation, and chromatographies on DEAE-cellulose and phosphocellulose. About 60 μg of recombinant S-II was obtained from 1 g of virus-infected cells. The molecular mass and specific activity of recombinant S-II were exactly the same as those of authentic S-II purified from Ehrlich ascites tumor cells.

AB - A cDNA for murine transcription factor S-II (Hirashima et al., J. Biol. Chem (1988) 263, 3858-3863) was inserted into a silkworm baculovirus vector and expressed in Bm-N cells, a cell line of Bombyx mori maintained in the laboratory. Recombinant S-II was purified from virus-infected cell extracts to near homogeneity by ammonium sulfate fractionation, and chromatographies on DEAE-cellulose and phosphocellulose. About 60 μg of recombinant S-II was obtained from 1 g of virus-infected cells. The molecular mass and specific activity of recombinant S-II were exactly the same as those of authentic S-II purified from Ehrlich ascites tumor cells.

UR - https://www.scopus.com/pages/publications/0024970462

UR - https://www.scopus.com/pages/publications/0024970462#tab=citedBy

U2 - 10.1016/S0006-291X(89)80115-9

DO - 10.1016/S0006-291X(89)80115-9

M3 - Article

C2 - 2730640

AN - SCOPUS:0024970462

SN - 0006-291X

VL - 160

SP - 1093

EP - 1099

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Production of functional S-II in Bombyx mori cells

Abstract

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