Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone

A. Adachi, H. E. Gendelman, S. Koenig, T. Folks, R. Willey, A. Rabson, M. A. Martin

    Research output: Contribution to journalArticlepeer-review

    2245 Scopus citations

    Abstract

    We constructed an infectious molecular clone of acquired immunodeficiency syndrome-associated retrovirus. Upon transfection, this clone directed the production of infectious virus particles in a wide variety of cells in addition to human T4 cells. The progeny, infectious virions, were synthesized in mouse, mink, monkey, and several human non-T cell lines, indicating the absence of any intracellular obstacle to viral RNA or protein production or assembly. During the course of these studies, a human colon carcinoma cell line, exquisitely sensitive to DNA transfection, was identified.

    Original languageEnglish (US)
    Pages (from-to)284-291
    Number of pages8
    JournalJournal of Virology
    Volume59
    Issue number2
    StatePublished - Nov 19 1986

    ASJC Scopus subject areas

    • Microbiology
    • Immunology
    • Insect Science
    • Virology

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