Prodan fluorescence mimics the GroEL folding cycle

Yogeet Kaur, Paul M. Horowitz

Research output: Contribution to journalArticlepeer-review

Abstract

The non-covalent fluorescent probe 6-propionyl-2-(dimethylamino) naphthalene sulfonate (prodan) binds to hydrophobic surfaces exposed on the surface of GroEL. Under identical experimental conditions free prodan exhibits a green emission peak of intensity 390,000 cps at 520 nm. However prodan bound to GroEL, GroEL-ATP, and GroEL-ATP-GroES shows emission peaks of intensities 500,000, 540,000, and 480,000 cps at 515, 512 and 515 nm, respectively, thus mimicing the way hydrophobic surfaces on GroEL become exposed during the folding cycle. Other hydrophobic probes like bis-ANS and dansyl lysine were unable to detect the minor changes in hydrophobic exposure of GroEL after it binds to ATP, although they were able to detect hydrophobic exposure in GroEL itself.

Original languageEnglish (US)
Pages (from-to)475-481
Number of pages7
JournalProtein Journal
Volume23
Issue number7
DOIs
StatePublished - Oct 2004
Externally publishedYes

Keywords

  • Bio-ANS
  • GroEL-ATP
  • Hydrophobic exposure
  • Prodan

ASJC Scopus subject areas

  • Analytical Chemistry
  • Bioengineering
  • Biochemistry
  • Organic Chemistry

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