Processivity of the DNA helicase activity of Escherichia coli recBCD enzyme

L. J. Roman, A. K. Eggleston, S. C. Kowalczykowski

Research output: Contribution to journalArticlepeer-review

92 Scopus citations

Abstract

A fluorescence assay was used to measure the processivity of Escherichia coli recBCD enzyme helicase activity. Under standard conditions, recBCD enzyme unwinds an average of 30 ± 3.2 kilobase pairs (kb)/DNA end before dissociating. The average processivity (P(obs)) of DNA unwinding under these conditions is 0.99997, indicating that the probability of unwinding another base pair is 30,000-fold greater than the probability of dissociating from the double-stranded DNA. The average number of base pairs unwound per binding event (N) is sensitive to both mono- and divalent salt concentration and ranges from 36 kb at 80 mM NaCl to 15 kb at 280 mM NaCl. The processivity of unwinding increases in a hyperbolic manner with increasing ATP concentration, yielding a K(N) value for ATP of 41 ± 9 μM and a limiting value of 32 ± 1.8 kb/end for the number of base pairs unwound. The importance of the processivity of recBCD enzyme helicase activity to the recBCD enzyme- dependent stimulation of recombination at Chi sites observed in vivo is discussed.

Original languageEnglish (US)
Pages (from-to)4207-4214
Number of pages8
JournalJournal of Biological Chemistry
Volume267
Issue number6
StatePublished - 1992

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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