Processing of DNA structures via DNA unwinding and branch migration by the S. cerevisiae Mph1 protein

Xiao Feng Zheng, Rohit Prakash, Dorina Saro, Simonne Longerich, Hengyao Niu, Patrick Sung

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

The budding yeast Mph1 protein, the putative ortholog of human FANCM, possesses a 3' to 5' DNA helicase activity and is capable of disrupting the D-loop structure to suppress chromosome arm crossovers in mitotic homologous recombination. Similar to FANCM, genetic studies have implicated Mph1 in DNA replication fork repair. Consistent with this genetic finding, we show here that Mph1 is able to mediate replication fork reversal, and to process the Holliday junction via DNA branch migration. Moreover, Mph1 unwinds 3' and 5' DNA Flap structures that bear key features of the D-loop. These biochemical results not only provide validation for a role of Mph1 in the repair of damaged replication forks, but they also offer mechanistic insights as to its ability to efficiently disrupt the D-loop intermediate.

Original languageEnglish (US)
Pages (from-to)1034-1043
Number of pages10
JournalDNA Repair
Volume10
Issue number10
DOIs
StatePublished - Oct 10 2011
Externally publishedYes

Keywords

  • Branch migration
  • D-loop
  • Fork regression
  • Helicase
  • Mph1
  • Recombination
  • Replication fork

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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