Primate homologues of rat TGN38: Primary structure, expression and functional implications

Sreenivasan Ponnambalam, Milena Girotti, Marie Laure Yaspo, Charles E. Owen, Anthony C F Perry, Tatsuo Suganuma, Tommy Nilsson, Mike Fried, George Banting, Graham Warren

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

cDNAs encoding the human and macaque homologues of rat TGN38 have been cloned and sequenced. The proteins have a highly conserved N terminus (comprising the signal peptide) and C terminus (comprising part of the lumenal domain, the membrane spanning region and cytoplasmic tail) but vary in the other part of the lumenal domain, which contains the repeat region. Whereas rat TGN38 contains 6 tandem repeats of an 8mer, both primate proteins possess 14 tandem repeats of a 14mer sequence. The human protein, like rat TGN38, is localised primarily to the TGN but is present on. the cell surface and returns via endosomes. This behaviour is consistent with conservation of the membrane spanning region and the cytoplasmic tail, which contain the retention and retrieval signals, respectively, for localisation in the TGN. The unexpected differences in the lumenal domain can best be rationalised by the fact that both types of repeat domains have most of the properties of mucins. We suggest that TGN38 homologues are mucin-like molecules that regulate membrane traffic to and from the TGN.

Original languageEnglish (US)
Pages (from-to)675-685
Number of pages11
JournalJournal of Cell Science
Volume109
Issue number3
StatePublished - Mar 1996
Externally publishedYes

Fingerprint

Primates
Tandem Repeat Sequences
Mucins
Membranes
Tail
Proteins
Endosomes
Macaca
Protein Sorting Signals
Complementary DNA
polypeptide C

Keywords

  • Endosome
  • Mucin
  • TGN38
  • TGN46
  • Trans-Golgi network

ASJC Scopus subject areas

  • Cell Biology

Cite this

Ponnambalam, S., Girotti, M., Yaspo, M. L., Owen, C. E., Perry, A. C. F., Suganuma, T., ... Warren, G. (1996). Primate homologues of rat TGN38: Primary structure, expression and functional implications. Journal of Cell Science, 109(3), 675-685.

Primate homologues of rat TGN38 : Primary structure, expression and functional implications. / Ponnambalam, Sreenivasan; Girotti, Milena; Yaspo, Marie Laure; Owen, Charles E.; Perry, Anthony C F; Suganuma, Tatsuo; Nilsson, Tommy; Fried, Mike; Banting, George; Warren, Graham.

In: Journal of Cell Science, Vol. 109, No. 3, 03.1996, p. 675-685.

Research output: Contribution to journalArticle

Ponnambalam, S, Girotti, M, Yaspo, ML, Owen, CE, Perry, ACF, Suganuma, T, Nilsson, T, Fried, M, Banting, G & Warren, G 1996, 'Primate homologues of rat TGN38: Primary structure, expression and functional implications', Journal of Cell Science, vol. 109, no. 3, pp. 675-685.
Ponnambalam S, Girotti M, Yaspo ML, Owen CE, Perry ACF, Suganuma T et al. Primate homologues of rat TGN38: Primary structure, expression and functional implications. Journal of Cell Science. 1996 Mar;109(3):675-685.
Ponnambalam, Sreenivasan ; Girotti, Milena ; Yaspo, Marie Laure ; Owen, Charles E. ; Perry, Anthony C F ; Suganuma, Tatsuo ; Nilsson, Tommy ; Fried, Mike ; Banting, George ; Warren, Graham. / Primate homologues of rat TGN38 : Primary structure, expression and functional implications. In: Journal of Cell Science. 1996 ; Vol. 109, No. 3. pp. 675-685.
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